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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/14421

Title: Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis
Other Titles: Research in Veterinary Science
Authors: Melo, Stella Maria Barrouin
Poester, Fernando Padilla
Ribeiro, Marcos Borges
Alcântara, Adriano Costa de
Aguiar, Paulo Henrique Palis
Nascimento, Ivana Lúcia de Oliveira
Schaer, Robert Eduard
Nascimento, Roberto José Meyer
Freire, Songelí Menezes
Keywords: Brucella canis;ELISA;Serodiagnosis;Canine brucellosis
Issue Date: 2007
Abstract: An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K = 0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve.
Description: Texto completo: acesso restrito. p. 340-346
URI: http://repositorio.ufba.br/ri/handle/ri/14421
ISSN: 0034-5288
Appears in Collections:Artigos Publicados em Periódicos (EMV)

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