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Title: HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates
Other Titles: Journal of Medical Virology
Authors: Miranda, Aline Cristina Mota
Araújo, Sérgio Pereira
Dias, Juarez Pereira
Colin, Denise Duizit
Kashima, Simone
Covas, Dimas Tadeu
Tavares-Neto, José
Castro, Bernardo Galvão
Alcântara, Luiz Carlos Júnior
Keywords: LTR region;Glycoproteins;Phylogenetic analysis;Protein sites
Issue Date: 2009
Abstract: To determine the seroprevalence of HTLV-1 in Brazil, and to review the virus molecular epidemiology in this Amazon population (Rio Branco-Acre), 219 blood donors were screened for HTLV-1. Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital Foundation (FUNDACRE). Neighbor-joining and Maximum Likelihood phylogenetic analyses of two (n = 2) complete LTR region sequences were performed with the PAUP* software. Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. Phylogenetic analysis demonstrated that the new isolate described in this study, and the unpublished LTR strain described in a previous report belong to the Transcontinental subgroup of the Cosmopolitan subtype, inside the Latin American cluster. A similar result was obtained when submitting, to the Automated Genotyping System, three LTR partial sequences from a previous study of the seroprevalence of HTLV-1 in the same Amazon population. In all analyzed env sequences, the potential protein site was found: two PKC phosphorylation sites at amino acid (aa) positions 310–312 and 342–344, one CK2 phosphorylation site at 194–197aa, three N-glycosylation sites at 222–225aa, 244–247aa and 272–275aa, and a single N-myristylation site at 327–338aa. In conclusion, potential protein sites described in HTLV-1 gp46 and gp21 confirm the presence of conserved sites in the HTLV-1 envelope proteins, likewise phylogenetic analysis suggests a possible recent introduction of the virus into North Brazil.
Description: Texto completo: acesso restrito. p. 1966-1971
URI: http://repositorio.ufba.br/ri/handle/ri/13601
ISSN: 0146-6615
Appears in Collections:Artigos Publicados em Periódicos (ICS)

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