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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/12965

Title: An imprint method for detecting leptospires in the hamster model of vaccine-mediated immunity for leptospirosis
Other Titles: Journal of Medical Microbiology
Authors: Chagas Júnior, Adenizar Delgado das
McBride, Alan J. A.
Athanazio, Daniel Abensur
Figueira, Cláudio P.
Medeiros, Marco A.
Reis, Mitermayer Galvão dos
Ko, Albert I.
McBride, Flávia W. C.
Issue Date: 2010
Abstract: In determining the efficacy of new vaccine candidates for leptospirosis, the primary end point is death and an important secondary end point is sterilizing immunity. However, evaluation of this end point is often hampered by the time-consuming demands and complexity of methods such as culture isolation (CI). In this study, we evaluated the use of an imprint (or touch preparation) method (IM) in detecting the presence of leptospires in tissues of hamsters infected with Leptospira interrogans serovar Copenhageni. In a dissemination study, compared to CI, the IM led to equal or improved detection of leptospires in kidney, liver, lung and blood samples collected post-infection and overall concordance was good (κ=0.61). Furthermore, in an evaluation of hamsters immunized with a recombinant leptospiral protein-based vaccine candidate and subsequently challenged, the agreement between the CI and IM was very good (κ=0.84). These findings indicate that the IM is a rapid method for the direct observation of Leptospira spp. that can be readily applied to evaluating infection in experimental animals and determining sterilizing immunity when screening potential vaccine candidates.
Description: Texto completo: acesso restrito. p.1632-1637
URI: http://www.repositorio.ufba.br/ri/handle/ri/12965
ISSN: 0022-2615
Appears in Collections:Artigos Publicados em Periódicos (ICS)

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