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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/11923

Title: Leishmanicidal activity of Cecropia pachystachya flavonoids: Arginase inhibition and altered mitochondrial DNA arrangement
Other Titles: Phytochemistry
Authors: Cruz, Ebenézer de Mello
Silva, Edson Roberto da
Maquiaveli, Claudia do Carmo
Alves, Eliomara Sousa Sobral
Lucon Junior, João Francisco
Reis, Matheus Balduino Gonçalves dos
Toledo, Cleyton Eduardo Mendes de
Cruz, Frederico Guare
Santos, Marcos André Vannier dos
Keywords: Cecropia pachystachya;Cecropiaceae;Arginase;Polyamines;Oxidative stress;Kinetoplast;Ultrastructure;Leishmania
Issue Date: 2013
Publisher: Phytochemistry
Abstract: The plant Cecropia pachystachya Trécul is widely used in Brazilian ethnomedicine to treat hypertension, asthma, and diabetes. Arginase is an enzyme with levels that are elevated in these disorders, and it is central to Leishmania polyamine biosynthesis. The aims of this study were to evaluate antileishmanial activity and inhibition of the arginase enzyme by C. pachystachya extracts, and to study changes in cellular organization using electron microscopy. The ethanol extract of C. pachystachya was tested on Leishmania (Leishmania) amazonensis promastigote survival/proliferation and arginase activity in vitro. Qualitative ultrastructural analysis was also used to observe changes in cell organization. The major bioactive molecules of the ethanol extract were characterized using liquid chromatography–electrospray ionizationmass spectrometry (LC–ESI-MS). The ethyl acetate fraction of the ethanol extract diminished promastigote axenic growth/survival, inhibited arginase activity, and altered a mitochondrial kinetoplast DNA (K-DNA) array. The bioactive compounds of C. pachystachya were characterized as glucoside flavonoids. Orientin (9) (luteolin-8-C-glucoside) was the main component of the methanol-soluble ethyl acetate fraction obtained from the ethanol extract and is an arginase inhibitor (IC50 15.9 lM). The ethyl acetate fraction was not cytotoxic to splenocytes at a concentration of 200 lg/mL. In conclusion, C. pachystachya contains bioactive compounds that reduce the growth of L. (L.) amazonensis promastigotes, altering mitochondrial K-DNA arrangement and inhibiting arginase.
Description: Texto completo. Acesso restrito. p. 71–77
URI: http://www.repositorio.ufba.br/ri/handle/ri/11923
ISSN: 0031-9422
Appears in Collections:Artigos Publicados em Periódicos (Quimica)

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