https://repositorio.ufba.br/handle/ri/8008
Tipo: | Artigo de Periódico |
Título: | Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment |
Título(s) alternativo(s): | Brazilian Journal of Microbiology |
Autor(es): | Oliveira, I. C. Almeida, Rogeria Comastri de Castro Hofer, Ernesto Almeida, Paulo Fernando de |
Autor(es): | Oliveira, I. C. Almeida, Rogeria Comastri de Castro Hofer, Ernesto Almeida, Paulo Fernando de |
Abstract: | A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 108 pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes. |
Palavras-chave: | Listeria bacteriophage A511 laser light detection skimmed milk |
Editora / Evento / Instituição: | Brazilian Journal of Microbiology |
URI: | http://www.repositorio.ufba.br/ri/handle/ri/8008 |
Data do documento: | 2012 |
Aparece nas coleções: | Artigo Publicado em Periódico (Escola de Nutrição) |
Arquivo | Descrição | Tamanho | Formato | |
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Bacteriophage amplification assay ......pdf | 194,23 kB | Adobe PDF | Visualizar/Abrir |
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