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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/7598
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dc.contributor.authorAtta, Ajax Mercês-
dc.contributor.authorAtta, Maria Luiza Brito de Sousa-
dc.contributor.authorD'Oliveira Junior, Argemiro-
dc.contributor.authorAlmeida, Roque Pacheco de-
dc.contributor.authorAraujo, Maria Ilma Andrade Santos-
dc.contributor.authorCarvalho Filho, Edgar Marcelino de-
dc.creatorAtta, Ajax Mercês-
dc.creatorAtta, Maria Luiza Brito de Sousa-
dc.creatorD'Oliveira Junior, Argemiro-
dc.creatorAlmeida, Roque Pacheco de-
dc.creatorAraujo, Maria Ilma Andrade Santos-
dc.creatorCarvalho Filho, Edgar Marcelino de-
dc.date.accessioned2012-12-17T15:34:45Z-
dc.date.available2012-12-17T15:34:45Z-
dc.date.issued2002-01-
dc.identifier.issn0074-0276-
dc.identifier.urihttp://www.repositorio.ufba.br/ri/handle/ri/7598-
dc.descriptionp. 101-103pt_BR
dc.description.abstractProcedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.pt_BR
dc.language.isoenpt_BR
dc.sourcehttp://dx.doi.org/10.1590/S0074-02762002000100017pt_BR
dc.subjectIgEpt_BR
dc.subjectIgG anti-IgEpt_BR
dc.subjectprotein Gpt_BR
dc.subjectvisceral leishmaniasispt_BR
dc.subjectschistosomiasis mansonipt_BR
dc.titleIgG anti-Ige autoantibodies in visceral leishmaniasispt_BR
dc.title.alternativeMemórias do Instituto Oswaldo Cruzpt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 97, n. 1pt_BR
Aparece nas coleções:Artigo Publicado em Periódico (FAR)

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