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dc.contributor.authorSilva, Tânia Regina Marques da-
dc.contributor.authorFreitas, Juliana Ribeiro de-
dc.contributor.authorSilva, Queilan Chagas-
dc.contributor.authorFigueira, Cláudio Pereira-
dc.contributor.authorRoxo, Eliana-
dc.contributor.authorLeão, Sylvia Cardoso-
dc.contributor.authorFreitas, Luiz Antônio Rodrigues de-
dc.contributor.authorVeras, Patrícia Sampaio Tavares-
dc.creatorSilva, Tânia Regina Marques da-
dc.creatorFreitas, Juliana Ribeiro de-
dc.creatorSilva, Queilan Chagas-
dc.creatorFigueira, Cláudio Pereira-
dc.creatorRoxo, Eliana-
dc.creatorLeão, Sylvia Cardoso-
dc.creatorFreitas, Luiz Antônio Rodrigues de-
dc.creatorVeras, Patrícia Sampaio Tavares-
dc.descriptionTexto completo: acesso restrito. p. 5628-5634pt_BR
dc.description.abstractThe virulence of different isolates of Mycobacterium has been associated with two morphologically distinguishable colonial variants: opaque (SmOp) and transparent (SmTr). In this report we used an in vitro assay to compare macrophage (Mφ) responses to SmOp and SmTr Mycobacterium fortuitum variants, taking advantage of the fact that these variants were derived from the same isolate. Cells preactivated or not with gamma interferon (IFN-γ) were infected with SmOp or SmTr M. fortuitum. We showed that SmOp and SmTr induced different levels of nitric oxide (NO) production by IFN-γ-stimulated Mφ. Indeed, the amount of IFN-γ-induced NO production by J774 cells was 4.8 to 9.0 times higher by SmOp (23.1 to 37.7 μM) compared to SmTr infection (3.9 to 4.8 μM) (P = 0.0332), indicating that virulent SmTr bacilli restricted NO production. In addition, IFN-γ-induced NO production by Mφ was higher when correlated with reduction of only avirulent SmOp bacillus viability. SNAP (S-nitroso-N-acetyl-dl-penicillamine) -induced NO production did not modify SmTr viability, indicating its resistance to nitrogen radicals. Electron microscopy studies were performed to evaluate the capacity of phagosomes to fuse with lysosomes labeled with bovine serum albumin-colloidal gold particles. By 24 h postinfection, 69% more phagosome-containing SmOp variant had fused with lysosomes compared to the SmTr-induced phagosomes. In conclusion, these data indicate that virulent SmTr bacilli may escape host defense by restricting IFN-γ-induced NO production, resisting nitrogen toxic radicals, and limiting phagosome fusion with lysosomes.pt_BR
dc.titleVirulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusionpt_BR
dc.title.alternativeInfection and Immunitypt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 70, n. 10pt_BR
Appears in Collections:Artigo Publicado em Periódico (Biologia)

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