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dc.contributor.authorMelo, Stella Maria Barrouin-
dc.contributor.authorLarangeira, Daniela Farias-
dc.contributor.authorSantos, Silvana Ornelas-
dc.contributor.authorChagas Júnior, Adenizar Delgado das-
dc.contributor.authorPaixão, Mariza-
dc.contributor.authorAguiar, Paulo Henrique Palis-
dc.contributor.authorSantos, Washington Luis Conrado dos-
dc.contributor.authorCarvalho, Lain Carlos Pontes de-
dc.creatorMelo, Stella Maria Barrouin-
dc.creatorLarangeira, Daniela Farias-
dc.creatorSantos, Silvana Ornelas-
dc.creatorChagas Júnior, Adenizar Delgado das-
dc.creatorPaixão, Mariza-
dc.creatorAguiar, Paulo Henrique Palis-
dc.creatorSantos, Washington Luis Conrado dos-
dc.creatorCarvalho, Lain Carlos Pontes de-
dc.description.abstractA method for the evaluation of splenic cellularity using samples collected by fine-needle aspirative biopsy was standardized in this work. The procedure includes erythrocyte lysing, preparation of cytospin films and staining by histochemical and immunocytochemical techniques. The cellular profiles of spleen preparations were compared with those observed in peripheral blood samples subjected to the same procedure. Two groups were compared, one consisting of 14 healthy uninfected and the other of 15 polysymptomatic Leishmania chagasi/infantum-infected dogs, from an endemic area for visceral leishmaniosis. Cell populations were identified by conventional hematoxilin–eosin and Wright’ stainings, and by immunocytochemistry using monoclonal antibodies against canine CD45RA and CD45RB, phagocytes and a pan-leukocyte antigen. Larger neutrophil (P < 0.0001) and monocyte/macrophage (P = 0.0036) relative counts and lower lymphocyte relative counts (P < 0.0001) were found in the spleen, and not in the blood, of the animals with leishmaniosis than in those of the healthy animals. The proportions of CD45RB+ cells were higher, and of CD45RA+ cells were lower, both in the spleen and in the blood of animals with leishmaniosis than in those of healthy dogs (P < 0.05). Additionally, hematoxilin–eosin-stained cytospins of spleen aspirates from Leishmania-infected animals permitted the easy visualization of amastigote forms inside phagocytes, under light microscopy.pt_BR
dc.publisherVeterinary Immunology and Immunopathologypt_BR
dc.subjectFine-needle aspirative biopsypt_BR
dc.subjectLeukocyte populationspt_BR
dc.subjectCanine visceral leishmaniosispt_BR
dc.titleA standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosispt_BR
dc.title.alternativeVeterinary Immunology and Immunopathologypt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv.111, n.3-4pt_BR
Appears in Collections:Artigo Publicado em Periódico (EMV)

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