Campo DC | Valor | Idioma |
dc.contributor.author | Cardoso, Luciana Santos | - |
dc.contributor.author | Araujo, Maria Ilma Andrade Santos | - |
dc.contributor.author | Góes, Alfredo M. | - |
dc.contributor.author | Pacífico, Lucila G. | - |
dc.contributor.author | Oliveira, Ricardo Riccio | - |
dc.contributor.author | Oliveira, Sergio C. | - |
dc.creator | Cardoso, Luciana Santos | - |
dc.creator | Araujo, Maria Ilma Andrade Santos | - |
dc.creator | Góes, Alfredo M. | - |
dc.creator | Pacífico, Lucila G. | - |
dc.creator | Oliveira, Ricardo Riccio | - |
dc.creator | Oliveira, Sergio C. | - |
dc.date.accessioned | 2012-06-29T14:02:29Z | - |
dc.date.available | 2012-06-29T14:02:29Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1475-2859 | - |
dc.identifier.uri | http://www.repositorio.ufba.br/ri/handle/ri/6259 | - |
dc.description.abstract | Background: Recombinant proteins expressed in Escherichia coli vectors are generally
contaminated with endotoxin. In this study, we evaluated the ability of Polymyxin B to neutralize
the effect of LPS present as contaminant on Schistosoma mansoni recombinant proteins produced
in E. coli in inducing TNF-α and IL-10. Peripheral blood mononuclear cells from individuals
chronically infected with S. mansoni were stimulated in vitro with recombinant Sm22.6, Sm14 and
P24 antigens (10 μg/mL) in the presence of Polymyxin B (10 μg/mL).
Results: The levels of cytokines were measured using ELISA. There was greater than 90 %
reduction (p < 0.05) in the levels of TNF-α and IL-10 when Polymyxin B was added to the cultures
stimulated with LPS. In cultures stimulated with S. mansoni recombinant proteins in the presence
of Polymyxin B, a reduction in the levels of TNF-α and IL-10 was also observed. However, the
percentage of reduction was lower when compared to the cultures stimulated with LPS, probably
because these proteins are able to induce the production of these cytokines by themselves.
Conclusion: This study showed that Polymyxin B was able to neutralize the effect of endotoxin,
as contaminant in S. mansoni recombinant antigens produced in E. coli, in inducing TNF-α and IL-10
production. | pt_BR |
dc.language.iso | en | pt_BR |
dc.publisher | Microbial Cell Factories | pt_BR |
dc.source | http://www.microbialcellfactories.com.ez10.periodicos.capes.gov.br/content/pdf/1475-2859-6-1.pdf | pt_BR |
dc.title | Polymyxin B as inhibitor of LPS contamination of Schistosoma mansoni recombinant proteins in human cytokine analysis | pt_BR |
dc.title.alternative | Microbial Cell Factories | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
Aparece nas coleções: | Artigo Publicado em Periódico (Faculdade de Medicina)
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