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dc.contributor.authorTeixeira, Márcia Cristina Aquino-
dc.contributor.authorOliveira, Geraldo Gileno de Sá-
dc.contributor.authorSantos, Patrícia Oliveira Meira-
dc.contributor.authorBahiense, Thiago Campanharo-
dc.contributor.authorSilva, Virginia Maria Goes da-
dc.contributor.authorRodrigues, Márcio Silva-
dc.contributor.authorLarangeira, Daniela Farias-
dc.contributor.authorSantos, Washington Luis Conrado dos-
dc.contributor.authorCarvalho, Lain Carlos Pontes de-
dc.creatorTeixeira, Márcia Cristina Aquino-
dc.creatorOliveira, Geraldo Gileno de Sá-
dc.creatorSantos, Patrícia Oliveira Meira-
dc.creatorBahiense, Thiago Campanharo-
dc.creatorSilva, Virginia Maria Goes da-
dc.creatorRodrigues, Márcio Silva-
dc.creatorLarangeira, Daniela Farias-
dc.creatorSantos, Washington Luis Conrado dos-
dc.creatorCarvalho, Lain Carlos Pontes de-
dc.description.abstractDomestic dogs are considered to be the main reservoirs of zoonotic visceral leishmaniasis. In this work, we evaluated a protocol to induce Leishmania infantum/Leishmania chagasi-specific cellular and humoral immune responses in dogs, which consisted of two injections of Leishmania promastigote lysate followed by a subcutaneous inoculation of viable promastigotes. The primary objective was to establish a canine experimental model to provide positive controls for testing immune responses to Leishmania in laboratory conditions. After inoculation of viable promastigotes, specific proliferative responses of peripheral blood mononuclear cells (PBMCs) to either Leishmania lysate or recombinant proteins, the in vitro production of interferon-γ by antigen-stimulated PBMCs and a significant increase in circulating levels of anti-Leishmania antibodies were observed. The immunized dogs also displayed positive delayed-type hypersensitivity reactions to Leishmania crude antigens and to purified recombinant proteins. An important finding that supports the suitability of the dogs as positive controls is that they remained healthy for the entire observation period, i.e., more than seven years after infection. Following the Leishmania antigen lysate injections, the infection of dogs by the subcutaneous route appears to induce a sustained cellular immune response, leading to an asymptomatic infection. This provides a useful model for both the selection of immunogenic Leishmania antigens and for immunobiological studies on their possible immunoprotective activities.pt_BR
dc.subjectLeishmania infantumpt_BR
dc.subjectLeishmania chagasipt_BR
dc.subjectcellular immune responsept_BR
dc.titleAn experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigenspt_BR
dc.title.alternativeMemórias do Instituto Oswaldo Cruzpt_BR
dc.typeArtigo de Periódicopt_BR
Appears in Collections:Artigo Publicado em Periódico (FAR)

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