Skip navigation
Universidade Federal da Bahia |
Repositório Institucional da UFBA
Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/5038
Tipo: Artigo de Periódico
Título: A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity
Título(s) alternativo(s): The Veterinary Journal
Autor(es): Aguiar, Paulo Henrique Palis
Melo, Stella Maria Barrouin
Franke, Carlos Roberto
Santos, Roberto Robson Borges dos
Silva, Tânia Maria Correia
Mengel, José O.
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
Autor(es): Aguiar, Paulo Henrique Palis
Melo, Stella Maria Barrouin
Franke, Carlos Roberto
Santos, Roberto Robson Borges dos
Silva, Tânia Maria Correia
Mengel, José O.
Santos, Washington Luis Conrado dos
Carvalho, Lain Carlos Pontes de
Abstract: This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of immune functions in dogs.
Palavras-chave: Canine
Leukocyte
Monoclonal antibodies
CD45
URI: http://www.repositorio.ufba.br/ri/handle/ri/5038
Data do documento: 2007
Aparece nas coleções:Artigo Publicado em Periódico (EMV)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Aguiar PH A monoclonal antibody against....pdf937,15 kBAdobe PDFVisualizar/Abrir
Mostrar registro completo do item Visualizar estatísticas


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.