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dc.creatorOliveira, Luiz Eduardo Lacerda de-
dc.date.accessioned2025-11-26T14:54:21Z-
dc.date.available2025-11-26T14:54:21Z-
dc.date.issued2025-08-15-
dc.identifier.citationOLIVEIRA, Luiz Eduardo Lacerda de. Inativação fotodinâmica de Enterococcus spp. por porfirinas catiônicas. Orientador: Ricardo Wagner Dias Portela. 2025. 88 f. il. Tese (Doutorado em Processos Interativos dos Órgãos e Sistemas) - Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, 2025.pt_BR
dc.identifier.urihttps://repositorio.ufba.br/handle/ri/43545-
dc.description.abstractIntroduction: Enterococcus are common human and animal’s intestinal commensals and can cause opportunistic infections. Reports of healthcare-associated infections (HAIs) caused by this bacterial genus have increased in recent years. Biofilm formation is the most important virulence factor in the establishment of these infections. Furthermore, strains obtained from both humans and animals are generally multidrug-resistant, which are associated with limited therapeutic options. Therefore, using photosensitizers, such as porphyrin, has gained importance as an alternative in controlling resistant strains. Objective: To evaluate the antimicrobial and antibiofilm activity of cationic porphyrins after photoactivation on Enterococcus spp. Material and methods: The porphyrins 3-H2TMeP+ and 4-H2TMeP+ were used, as well as 25 Enterococcus strains, including three references and 22 clinical isolates from humans (from dental canals) and animals (dog, cat, goat and snake). The species studied included E. faecalis, E. faecium, E. casseliflavus, E. gallinarum, and E. hirae. The MIC100 and MBC100 were determined by broth microdilution tests, following the CLSI M07-A9 (2012) standard. Assays with reactive oxygen species (ROS) scavengers were also performed to identify which ROS are involved in photoinactivation, and atomic force microscopy (AFM) was used to assess cellular changes after treatment with the photoactivated porphyrin. Results: The results indicated that antimicrobial photodynamic therapy (aPDT) with the tested cationic porphyrins was effective, , exerting a bactericidal effect at micro-concentrations (0.27 to 8.75 µM) against Enterococcus spp. strains tested. Both porphyrins showed antibiofilm activity under light, with greater activity at concentrations up to 4.38 µM. Data suggests the activity of type I and II oxidative pathways in E. faecalis and E. faecium. AFM analysis revealed a significant reduction in adhesion energy and changes in mechanical stiffness, accompanied by topographic disorganization and heterogeneity on the bacterial surface. Conclusion: Antimicrobial photodynamic therapy using cationic porphyrins showed promising results in inactivating of Enterococcus spp., leading to changes in surfaces’ adhesion, which tends to reduce colonization and biofilm formation. Thus, this suggests that aPDT may be a possible alternative in fighting against microbial resistance, especially in infections associated with biofilms.pt_BR
dc.languageporpt_BR
dc.publisherUniversidade Federal da Bahiapt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBiofilmespt_BR
dc.subjectEspécies reativas de oxigêniopt_BR
dc.subjectTerapia fotodinâmica antimicrobiana (aPDT)pt_BR
dc.subjectResistência a antimicrobianospt_BR
dc.subject.otherBiofilmspt_BR
dc.subject.otherReactive oxygen species (ROS)pt_BR
dc.subject.otherAntimicrobial photodynamic therapy (aPDT)pt_BR
dc.subject.otherAntimicrobial Drug Resistancept_BR
dc.titleInativação fotodinâmica de Enterococcus spp. por porfirinas catiônicaspt_BR
dc.title.alternativePhotodynamic inactivation of Enterococcus spp. by cationic porphyrinspt_BR
dc.typeTesept_BR
dc.publisher.programPrograma de Pós-Graduação em Processos Interativos dos Órgãos e Sistemas (PPGORGSISTEM) pt_BR
dc.publisher.initialsUFBApt_BR
dc.publisher.countryBrasilpt_BR
dc.subject.cnpqCNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA MEDICApt_BR
dc.contributor.advisor1Portela, Ricardo Wagner Dias-
dc.contributor.advisor1IDhttps://orcid.org/0000-0001-9095-776Xpt_BR
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/3118063347109613pt_BR
dc.contributor.referee1Kobayashi, Renata Katsuko Takayama-
dc.contributor.referee1IDhttps://orcid.org/0000-0001-7170-2155pt_BR
dc.contributor.referee1Latteshttp://lattes.cnpq.br/3188392520162374pt_BR
dc.contributor.referee2Rezende, Cintia Silva Minafra e-
dc.contributor.referee2IDhttps://orcid.org/0000-0002-0727-5651pt_BR
dc.contributor.referee2Latteshttp://lattes.cnpq.br/5841210447886226pt_BR
dc.contributor.referee3Bittencourt, Marta Vasconcelos-
dc.contributor.referee3Latteshttp://lattes.cnpq.br/9699593744135578pt_BR
dc.contributor.referee4Antón, Ana Rita Sokolonski-
dc.contributor.referee4IDhttps://orcid.org/0000-0001-8089-4128pt_BR
dc.contributor.referee4Latteshttp://lattes.cnpq.br/1228384941765961pt_BR
dc.contributor.referee5Portela, Ricardo Wagner Dias-
dc.contributor.referee5IDhttps://orcid.org/0000-0001-9095-776Xpt_BR
dc.contributor.referee5Latteshttp://lattes.cnpq.br/3118063347109613pt_BR
dc.creator.IDhttps://orcid.org/0000-0002-9756-8633pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/9342223034690726pt_BR
dc.description.resumoIntrodução: Enterococcus são comensais intestinais comuns em animais e em humanos, que podem causar infecções oportunistas. Nos últimos anos, aumentaram os relatos de infecções relacionadas à assistência à saúde (IRAS) por esse gênero bacteriano. A formação de biofilme é o fator de virulência mais importante no estabelecimento dessas infecções. Além disso, as cepas obtidas tanto de humanos quanto de animais são geralmente multirresistentes, o que vem limitando as opções terapêuticas. Dessa forma, o uso de fotossensibilizadores, como a porfirina, tem ganhado importância como alternativa no controle de cepas resistentes. Objetivo: Avaliar a atividade antimicrobiana e antibiofilme de porfirinas catiônicas, após fotoativação, sobre Enterococcus spp. Material e métodos: Foram usadas as porfirinas 3-H2TMeP+ e 4-H2TMeP+, e 25 cepas de Enterococcus, sendo 3 referências e 22 isolados clínicos de humanos (canal dental) e de animais (cão, gato, cabra e serpente). Entre as espécies estudadas estão E. faecalis, E. faecium, E. casseliflavus, E. gallinarum e E. hirae. Por testes de microdiluição em caldo, seguindo a norma CLSI M07-A9 (2012), foram determinadas a MIC100 e a MBC100. Ensaios com sequestradores de espécies reativas de oxigênio (ROS) também foram realizados para verificar a espécie que atua na fotoinativação, assim como microscopia de força atômica (AFM) para verificar as alterações celulares após tratamento com a porfirina fotoativada. Resultados: Os resultados indicaram que a terapia fotodinâmica antimicrobiana (aPDT) com as porfirinas catiônicas testadas foi eficaz, tendo efeito bactericida em microconcentrações (0,27 a 8,75 µM) nas cepas de Enterococcus spp. testadas. Ambas porfirinas mostraram efeito antibiofilme sob luz, com maior ação até 4,38 µM. Os dados sugerem a atividade das vias oxidativas do tipo I e II em E. faecalis e em E. faecium. A análise de AFM revelou redução significativa da energia de adesão e alteração na rigidez mecânica, acompanhadas por desorganização topográfica e heterogeneidade na superfície bacteriana. Conclusão: A terapia fotodinâmica antimicrobiana com utilização de porfirinas catiônicas mostrou-se promissora na inativação de Enterococcus spp., levou à alteração da adesão a superfícies, o que tende a reduzir a colonização e a formação de biofilmes, sugerindo, dessa forma, que a aPDT pode ser uma possível alternativa na luta contra a resistência microbiana, especialmente em infecções associadas a biofilmes.pt_BR
dc.publisher.departmentInstituto de Ciências da Saúde - ICSpt_BR
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