Efeito da proteína hidrolisada de frango sobre a digestibilidade, fermentação intestinal e marcadores da função renal e cardíaca de cães

Abstract

Hydrolyzed poultry by-product meal (HPM) has been used as a functional ingredient in pet food, as it has proteins with lower molecular weight, high digestibility and peptides that have antihypertensive properties, which are capable of inhibiting the activity of angiotensin- converting enzyme (ACE). This research involved 2 studies. The first study aimed to evaluate the inclusion of 10% HPM in the diet of dogs, replacing conventional poultry by-product meal (CPM), on the digestibility of nutrients and on the long-term consumption on serum ACE activity, aldosterone concentration and functioning of the cardiovascular and renal systems. Two diets were used (CPM and HPM), 8 dogs per diet and evaluated for 120 days. The digestibility of dry matter, organic matter, ether extract, crude protein, nitrogen-free extract, crude fiber and metabolizable energy were determined by the total feces collection method without urine collection. At days 0, 60 and 120 of consumption, blood and urine were collected to evaluate ACE, aldosterone and renal function; and an echocardiogram and electrocardiogram were performed. The second study evaluated the efficacy of HPM in inhibiting ACE, applying a protocol to 5 healthy dogs with renin-angiotensin-aldosterone system (RAAS) pharmacologically activated by furosemide, lasting 32 days. Data were evaluated by ANOVA using SigmaPlot v.12.0 at 5% probability. As results of the first study, nutrient digestibility and metabolizable energy were similar between CPM and HPM treatments (p>0.05). There was no effect of the diets on the cardiac parameters evaluated (p>0.05). There was a time effect, but no diet effect, for serum ACE, aldosterone, creatinine and potassium; and fractional excretion of sodium and potassium, however values remained within the reference range. The urinary aldosterone:creatinine did not change over time. As a result of the second study, PHF at 10% inclusion was not able to keep inhibition oft ACE in dogs with activated RAAS. The inclusion of 10% PHF in extruded diets for dogs and its consumption for 120 days does not promote adverse effects on the renal or cardiovascular systems and nutrient digestibility, in addition to not promoting changes in the RAAS and the health of dogs.