Avaliação da capacidade imunomoduladora do antígeno recombinante rSm29 na hanseníase

Abstract

Leprosy is a chronic, insidious infectious disease that is difficult to treat, especially in its reactional forms, which has prompted the development of new drugs, especially immunobiologicals for its treatment. The antigen (rSm29) has been shown to have an immunoregulatory function, by immunoregulatory function, through the induction of IL-10 and capable of modulating inflammatory responses with a Th1 and Th2 profile, demonstrated in diseases such as asthma, leishmaniasis and HTLV-1. In order to understand how this antigen might behave in leprosy, this study sought to evaluate the modulatory role of rSm29 in the disease. A cross-sectional pilot study was carried out with 17 leprosy patients. After blood collection, the PSMCs were separated and incubated for 72 hours in four groups categorized according to different stimuli: No stimulus, M. leprae, M. leprae+rSm29 and rSm29. RNA was then extracted using TRIzol, converted to cDNA and PCR conversion to cDNA and real-time PCR (qRT-PCR) using the Taqman® method for the target genes (TLR2, TLR4, IL-10 and TNF) and the Bioplex® immunoassay to measure important immunological markers of the disease. Gene expression of the TLR2 gene was higher in the group co-stimulated with M. leprae + rSm29 compared to M. leprae and significant when compared to the non-stimulated group (medium) (p = 0.0317). On the other hand On the other hand, the expression of TLR4 was higher in cultures in the presence of the M. leprae antigen compared to unstimulated (p = 0.0159) and co-stimulated (p = 0.0317) cultures. We also observed that the expression of the IL10 gene was higher in cultures in the presence of the antigens and presence of the antigens and significant when comparing M. leprae + rSm29 with cultures (p = 0.0159). There was no significant variation in TNF expression between the groups. between the groups. The results show significant differences in concentrations of the markers in the supernatants co-stimulated with the two antigens compared to M. leprae sonicated in the PB group observed in G-CSF, GM-CSF, IFN-γ, TNF-α. 17.In addition to the significant differences observed in the mediators of the response mediators, the cytokines and chemokines IL-1β, IL-2, IL-4, IL-5, IL-, significant differences were also observed in the stimulation of the PB group. a significant difference was also observed when stimulated with the recombinant antigen alone versus the antigen alone versus the M. leprae + rSm29 condition in the PB group, the addition of the rSm29 antigen to M. leprae in a culture of leprosy patients contributed to the development of a Th1-type cellular response. This response could help leprosy patients to better control their mycobacterial infection, thus favoring the milder form of the disease.