Estudo termográfico e histopatológico dos efeitos da terapia do jato de plasma no processo de reparo tecidual em ratos

Abstract

Biomodulatory therapies have been used with the aim of improving the healing pattern and assisting in the tissue repair process. The plasma jet is an electrotherapeutic resource that is currently being highly recommended for dermatological and aesthetic use, with the aim of treating wrinkles and expression lines, stretch marks and acne scarring. In addition, studies have confirmed its bactericidal action, a fact that can expand its use for other therapeutic purposes. Objective: To evaluate the effects of plasma jet in the repair of standardized surgical wounds in rat skin through infrared thermographic imaging and histopathological analysis. Methodology: A total of 20 animals were used, subdivided into two groups - the control group (GC) and the group treated with the plasma jet (GJP). A standardized wound was made on the back of the animals, and thermographic recording was performed at different periods. The control group did not undergo any type of therapy, whereas in the GJP plasma jet therapy was performed (conduction mode, direct current, intensity of 5/8 and time of 90 seconds), immediately after surgery, and on the two days Next. Half of the animals in each group were sacrificed on the 5th day after the surgical procedure, and the rest on the 10th day. A tissue sample was removed from each animal for processing and histological analysis. Statistical analysis was performed. Result: The thermographic analysis showed a higher Delta T in the group treated with plasma jet, especially on the 5th day (p>0.05), these results corroborate the findings in the histological analysis. Plasma jet treatment was able to promote an improvement in the pattern of collagen fibers in animals treated with the technique, suggesting an increase in the stimulation of fibroblast activity in the proliferative phase. Conclusion: The present study found that the plasma jet represents a promising and adjuvant tool, capable of helping to control inflammation and favor the healing process.