https://repositorio.ufba.br/handle/ri/25928
Tipo: | Artigo de Periódico |
Título: | Amburana cearensis seed extracts protect PC-12 cells against toxicity induced by glutamate |
Autor(es): | Pereira, Erica Patricia Lima Souza, Suzana Braga de Santos, Cleonice Creusa dos Santos, Leticia O. Cerqueira, Martins Dias de Fernandez, Luzimar Gonzaga Silva, Victor D. A. Costa, Silvia L. Ribeiro, Paulo R. |
Autor(es): | Pereira, Erica Patricia Lima Souza, Suzana Braga de Santos, Cleonice Creusa dos Santos, Leticia O. Cerqueira, Martins Dias de Fernandez, Luzimar Gonzaga Silva, Victor D. A. Costa, Silvia L. Ribeiro, Paulo R. |
Abstract: | Amburana cearensis (Allemão) A.C. Sm., Fabaceae, has been widely studied for its medicinal activities. Many neurodegenerative disorders are caused by oxidative stress, mitochondrial dysfunction, excitotoxicity induced by glutamate and ultimately cell death. This study describes the chemical profile of the ethanolic, hexane, dichloromethane, and ethyl acetate extracts obtained from seeds of A. cearensis. The objective of this study was to investigate the chemical profile of extracts obtained from seeds of A. cearensis, as well as their cytotoxicity and neuroprotective effects in cultures of neural PC12 cells. Metabolite profile was performed by GC–MS. PC12 cells were treated with increasing concentrations of the extracts (0.01–2000 g/ml) and the cell viability was analyzed after 24 and 72 h using an MTT test. For the excitotoxicity assay, PC12 cells were pre-treated with glutamate (1 mM) for 6 h and treated with increasing concentrations (0.1–1000 g/ml) of the extracts. The chromatographic analysis of the extracts detected various compounds with antioxidant properties, with the majority of peaks corresponding to the isoflavone coumarin. Only the hexane extract showed toxicity after 72 h exposure at the highest concentration (1000 g/ml). By contrast, all extracts increased the cellular viability of PC12 cells against the toxicity caused by glutamate. Therefore,the extracts from the seeds of A. cearensis showed no toxicity and have neuroprotective potential against neuronal damage induced by glutamate, which may be related to their antioxidant properties. © 2016 Sociedade Brasileir |
Palavras-chave: | Amburana cearensis Glutamate PC12 cells Excitotoxicity |
País: | Brasil |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/25928 |
Data do documento: | 4-Mai-2018 |
Aparece nas coleções: | Artigo Publicado em Periódico (Renorbio) |
Arquivo | Descrição | Tamanho | Formato | |
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AMBURANA CEARENSIS SEED EXTRACTS PROTECT PC-12 CELLS AGAINST TOXICITY INDUCED BY GLUTAMATE.pdf | 809,96 kB | Adobe PDF | Visualizar/Abrir |
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