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https://repositorio.ufba.br/handle/ri/20341| Tipo: | Artigo de Periódico |
| Título: | Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| Título(s) alternativo(s): | Revista de Ciências Médicas e Biológicas |
| Autor(es): | Couto, Alessanda Abdo do Barros, José Júnior França de Couceiro, José Nelson dos Santos Silva |
| Autor(es): | Couto, Alessanda Abdo do Barros, José Júnior França de Couceiro, José Nelson dos Santos Silva |
| Abstract: | Influenza virus sialidase develops an essential activity on cellular glycoproteins, then permitting the dissemination of the virus infections by preventing virus-virus self aggregation and virus-cell rebinding. Two purified variant samples of influenza A/Memphis/102/72 (H3N2) viruses, which are recognized for their receptor-binding activity to a-2,6 or a- 2,3-sialyllactose structures, were analysed for their sialidase activity on different natural and artificial substrates. The M1/ 5 sample exhibited higher sialidase activity on fetuin (O.D.=0.226), MPN (O.D.=0.110) and human erythrocytes (10,240 HAU/ml), while the activity of the M1/5HS8 sample on these substrates was expressed by O.D.=0.129, O.D.=0.065 and 2,560 HAU/ml when using fetuin, MPN and human erythrocytes as substrates, respectively. However, the M1/5HS8 sample showed more significative sialidase activity on mucin when compared to the M1/5 sample: the enzyme activity of first sample was responsible for liberation of 3.5 nmol of free sialic acids while the last one produced 16.5 nmol of free sialic acids |
| Palavras-chave: | Influenza virus. Receptor-binding variants. Neuraminidase (NA). Natural and artificial substrates. |
| Editora / Evento / Instituição: | Instituto de Ciências da Saúde/ Universidade Federal da Bahia |
| Citação: | COUTO, A. A. do. Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 13-19, jan./jun. 2004. |
| Tipo de Acesso: | Acesso Aberto |
| URI: | http://repositorio.ufba.br/ri/handle/ri/20341 |
| Data do documento: | 2004 |
| Aparece nas coleções: | Artigo Publicado em Periódico (PPGPIOS) |
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| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| R. Ci. méd. biol., v.3, n. 1-2004.pdf_0 | 66,88 kB | Adobe PDF | Visualizar/Abrir |
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