Campo DC | Valor | Idioma |
dc.contributor.author | Hughes, J. B. | - |
dc.contributor.author | Sousa, J. S. | - |
dc.contributor.author | Barreto, R. A. | - |
dc.contributor.author | Silva, A. R. | - |
dc.contributor.author | Souza, C. S. | - |
dc.contributor.author | Silva, V. D. A. | - |
dc.contributor.author | Silva, B. M. P. | - |
dc.contributor.author | Freitas, S. R. V. B. | - |
dc.contributor.author | Costa, Maria de Fátima Dias | - |
dc.contributor.author | El-Bachá, Ramon dos Santos | - |
dc.contributor.author | Batatinha, Maria José Moreira | - |
dc.contributor.author | Tardy, Marcienne Bloch | - |
dc.contributor.author | Velozo, Eudes da Silva | - |
dc.contributor.author | Costa, Silvia Lima | - |
dc.creator | Hughes, J. B. | - |
dc.creator | Sousa, J. S. | - |
dc.creator | Barreto, R. A. | - |
dc.creator | Silva, A. R. | - |
dc.creator | Souza, C. S. | - |
dc.creator | Silva, V. D. A. | - |
dc.creator | Silva, B. M. P. | - |
dc.creator | Freitas, S. R. V. B. | - |
dc.creator | Costa, Maria de Fátima Dias | - |
dc.creator | El-Bachá, Ramon dos Santos | - |
dc.creator | Batatinha, Maria José Moreira | - |
dc.creator | Tardy, Marcienne Bloch | - |
dc.creator | Velozo, Eudes da Silva | - |
dc.creator | Costa, Silvia Lima | - |
dc.date.accessioned | 2011-07-18T20:05:17Z | - |
dc.date.available | 2011-07-18T20:05:17Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1519-9940 | - |
dc.identifier.uri | http://www.repositorio.ufba.br/ri/handle/ri/1899 | - |
dc.description | p. 31-41 | pt_BR |
dc.description.abstract | Prosopis juliflora is largely used for feeding dairy and
beef cattle. However, this plant has been found to
cause neurotoxic disease when it is the sole sustenance
for the animal. In the central nervous system (CNS),
the glial cells are essential in maintaining neuronal cell
health and integrity. In this study, an extract containing
alkaloids (AE) from P. juliflora pods was obtained and
tested on glial cells of the lineage GL-15 to investigate:
cell cytotoxicity, morphological changes and the
expression of GFAP, a marker of astrocytic reactivity.
Concentrations between 0.03-30μg/ml of AE were
assayed for 24-72h. The MTT test, LDH activity, and
Trypan blue assay revealed dose-dependant
cytotoxicity for GL-15 cells. Growth curves designed
using Trypan blue staining displayed significant
reduction (p<0.05) of the number of viable GL-15 cells, which reached maximum effect at 30μg/ml of
AE. Immunocytochemistry and western blotting assay
performed for the cytoskeletal proteins, vimentin and
GFAP, revealed expression of vimentin in GL-15 cells
at the lowest concentration (0.03μg/ml), and a change
on their morphology together with an increase of the
number of the GFAP positive cells. These effects were
not detected when the highest concentrations (3-
30μg/ml) were tested. The results suggest that the
extract containing alkaloids may be involved in the phenotypical modifications detected on the lineage
studied, in the expression of the cytoskeletal proteins,
and in the reduction of the viable cell number. | pt_BR |
dc.language.iso | en | pt_BR |
dc.subject | Prosopis juliflora | pt_BR |
dc.subject | alkaloids | pt_BR |
dc.subject | cytotoxicity | pt_BR |
dc.subject | glia | pt_BR |
dc.title | Cytotoxic effects of an extract containing alkaloids obtained from Prosopis juliflora Sw. D.C. (Algaroba) pods on glioblastoma cells | pt_BR |
dc.title.alternative | Revista Brasileira de Saúde e Produção Animal | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.description.localpub | Salvador | pt_BR |
dc.identifier.number | v. 6, n. 1 | pt_BR |
Aparece nas coleções: | Artigo Publicado em Periódico (EMV)
|