Skip navigation
Universidade Federal da Bahia |
Repositório Institucional da UFBA
Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/17873
Tipo: Artigo de Periódico
Título: Strongyloidiasis and Infective Dermatitis Alter Human T Lymphotropic Virus-1 Clonality in vivo
Título(s) alternativo(s): PLoS ONE
Autor(es): Gillet, Nicolas A.
Cook, Lucy
Laydon, Daniel J.
Hlela, Carol
Verdonck, Kristien
Alvarez, Carolina
Gotuzzo, Eduardo
Clark, Daniel
Farré, Lourdes
Bittencourt, Achilea Candida Lisboa
Asquith, Becca
Taylor, Graham P.
Bangham, Charles R. M.
Autor(es): Gillet, Nicolas A.
Cook, Lucy
Laydon, Daniel J.
Hlela, Carol
Verdonck, Kristien
Alvarez, Carolina
Gotuzzo, Eduardo
Clark, Daniel
Farré, Lourdes
Bittencourt, Achilea Candida Lisboa
Asquith, Becca
Taylor, Graham P.
Bangham, Charles R. M.
Abstract: Human T-lymphotropic Virus-1 (HTLV-1) is a retrovirus that persists lifelong by driving clonal proliferation of infected T-cells. HTLV-1 causes a neuroinflammatory disease and adult T-cell leukemia/lymphoma. Strongyloidiasis, a gastrointestinal infection by the helminth Strongyloides stercoralis, and Infective Dermatitis associated with HTLV-1 (IDH), appear to be risk factors for the development of HTLV-1 related diseases. We used high-throughput sequencing to map and quantify the insertion sites of the provirus in order to monitor the clonality of the HTLV-1-infected T-cell population (i.e. the number of distinct clones and abundance of each clone). A newly developed biodiversity estimator called “DivE” was used to estimate the total number of clones in the blood. We found that the major determinant of proviral load in all subjects without leukemia/lymphoma was the total number of HTLV-1-infected clones. Nevertheless, the significantly higher proviral load in patients with strongyloidiasis or IDH was due to an increase in the mean clone abundance, not to an increase in the number of infected clones. These patients appear to be less capable of restricting clone abundance than those with HTLV-1 alone. In patients co-infected with Strongyloides there was an increased degree of oligoclonal expansion and a higher rate of turnover (i.e. appearance and disappearance) of HTLV-1-infected clones. In Strongyloides co-infected patients and those with IDH, proliferation of the most abundant HTLV-1+ T-cell clones is independent of the genomic environment of the provirus, in sharp contrast to patients with HTLV-1 infection alone. This implies that new selection forces are driving oligoclonal proliferation in Strongyloides co-infection and IDH. We conclude that strongyloidiasis and IDH increase the risk of development of HTLV-1-associated diseases by increasing the rate of infection of new clones and the abundance of existing HTLV-1+ clones.
País: Brasil
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/17873
Data do documento: 2013
Aparece nas coleções:Artigo Publicado em Periódico (Faculdade de Medicina)

Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
Nicolas A. Gillet.pdf1,03 MBAdobe PDFVisualizar/Abrir
Mostrar registro completo do item Visualizar estatísticas


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.