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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/16380
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dc.contributor.authorDrexler, Jan Felix-
dc.contributor.authorGeipel, Andreas-
dc.contributor.authorKönig, Alexander-
dc.contributor.authorCorman, Victor Max-
dc.contributor.authorRiel, Debby van-
dc.contributor.authorCarneiro, Aroldo José Borges-
dc.contributor.authorFranke, Carlos Roberto-
dc.creatorDrexler, Jan Felix-
dc.creatorGeipel, Andreas-
dc.creatorKönig, Alexander-
dc.creatorCorman, Victor Max-
dc.creatorRiel, Debby van-
dc.creatorCarneiro, Aroldo José Borges-
dc.creatorFranke, Carlos Roberto-
dc.date.accessioned2014-10-10T15:08:08Z-
dc.date.issued2013-
dc.identifier.issn1091-6490-
dc.identifier.urihttp://repositorio.ufba.br/ri/handle/ri/16380-
dc.descriptionTexto completo: acesso restrito. p. 16151–16156pt_BR
dc.description.abstractThe hepatitis B virus (HBV), family Hepadnaviridae, is one of most relevant human pathogens. HBV origins are enigmatic, and no zoonotic reservoirs are known. Here, we screened 3,080 specimens from 54 bat species representing 11 bat families for hepadnaviral DNA. Ten specimens (0.3%) from Panama and Gabon yielded unique hepadnaviruses in coancestral relation to HBV. Full genome sequencing allowed classification as three putative orthohepadnavirus species based on genome lengths (3,149–3,377 nt), presence of middle HBV surface and X-protein genes, and sequence distance criteria. Hepatic tropism in bats was shown by quantitative PCR and in situ hybridization. Infected livers showed histopathologic changes compatible with hepatitis. Human hepatocytes transfected with all three bat viruses cross-reacted with sera against the HBV core protein, concordant with the phylogenetic relatedness of these hepadnaviruses and HBV. One virus from Uroderma bilobatum, the tent-making bat, cross-reacted with monoclonal antibodies against the HBV antigenicity determining S domain. Up to 18.4% of bat sera contained antibodies against bat hepadnaviruses. Infectious clones were generated to study all three viruses in detail. Hepatitis D virus particles pseudotyped with surface proteins of U. bilobatum HBV, but neither of the other two viruses could infect primary human and Tupaia belangeri hepatocytes. Hepatocyte infection occurred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be neutralized by sera from vaccinated humans. Antihepadnaviral treatment using an approved reverse transcriptase inhibitor blocked replication of all bat hepadnaviruses. Our data suggest that bats may have been ancestral sources of primate hepadnaviruses. The observed zoonotic potential might affect concepts aimed at eradicating HBV.pt_BR
dc.language.isoenpt_BR
dc.rightsAcesso Abertopt_BR
dc.sourcehttp://dx.doi.org/10.1073/pnas.1308049110pt_BR
dc.subjectEvolutionpt_BR
dc.subjectZoonosispt_BR
dc.subjectViromept_BR
dc.subjectMetagenomicspt_BR
dc.subjectReverse geneticspt_BR
dc.titleBats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytespt_BR
dc.title.alternativeProceedings of the National Academy of Sciencespt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 110, n. 40pt_BR
dc.embargo.liftdate10000-01-01-
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