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dc.contributor.authorRodrigues, Cleusa Alves Theodoro-
dc.contributor.authorBatista, Luís Fábio da Silva-
dc.contributor.authorTeixeira, Márcia Cristina Aquino-
dc.contributor.authorPereira, Andréa Mendes-
dc.contributor.authorSantos, Patrícia Oliveira Meira-
dc.contributor.authorOliveira, Geraldo Gileno de Sá-
dc.contributor.authorFreitas, Luiz Antônio Rodrigues de-
dc.contributor.authorVeras, Patrícia Sampaio Tavares-
dc.creatorRodrigues, Cleusa Alves Theodoro-
dc.creatorBatista, Luís Fábio da Silva-
dc.creatorTeixeira, Márcia Cristina Aquino-
dc.creatorPereira, Andréa Mendes-
dc.creatorSantos, Patrícia Oliveira Meira-
dc.creatorOliveira, Geraldo Gileno de Sá-
dc.creatorFreitas, Luiz Antônio Rodrigues de-
dc.creatorVeras, Patrícia Sampaio Tavares-
dc.descriptionTexto completo: acesso restrito. p.197–205pt_BR
dc.description.abstractLeishmania chagasi is the causative agent of visceral leishmaniasis in both humans and dogs in the New World. The dog is the main domestic reservoir and its infection displays different clinical presentations, from asymptomatic to severe disease. Macrophages play an important role in the control of Leishmania infection. Although it is not an area of intense study, some data suggest a role for canine macrophages in parasite killing by a NO-dependent mechanism. It has been proposed that control of human disease could be possible with the development of an effective vaccine against canine visceral leishmaniasis. Development of a rapid in vitro test to predict animal responses to Leishmania infection or vaccination should be helpful. In this study, an in vitro model was established to test whether peripheral blood mononuclear cell (PBMC) supernatants from dogs immunized with promastigote lysates and infected with L. chagasi promastigotes could stimulate macrophages from healthy dogs in order to control parasite infection. PBMC from a majority of the immunized and experimentally infected dogs expressed IFN-γ mRNA and secreted IFN-γ when stimulated with soluble L. chagasi antigen (SLA) in vitro. Additionally, the supernatants from stimulated PBMC were able to reduce the percentage of infected donor macrophages. The results also indicate that parasite killing in this system is dependent on NO, since aminoguanidine (AMG) reversed this effect. This in vitro test appears to be useful for screening animal responses to parasite inoculation as well as studying the lymphocyte effector mechanisms involved in pathogen killing by canine macrophages.pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectLeishmania chagasipt_BR
dc.subjectDog macrophagept_BR
dc.subjectParasite killingpt_BR
dc.subjectScreening in vitro testpt_BR
dc.titlePeripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitropt_BR
dc.title.alternativeVeterinary Parasitologypt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 143, n. 3-4pt_BR
Appears in Collections:Artigo Publicado em Periódico (EMV)

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