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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/15925
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dc.contributor.authorCarvalho, Augusto M.-
dc.contributor.authorMagalhães, Andréa-
dc.contributor.authorCarvalho, Lucas Pedreira de-
dc.contributor.authorBacellar, Maria Olívia Amado Ramos-
dc.contributor.authorScott, Phillip-
dc.contributor.authorCarvalho Filho, Edgar Marcelino de-
dc.creatorCarvalho, Augusto M.-
dc.creatorMagalhães, Andréa-
dc.creatorCarvalho, Lucas Pedreira de-
dc.creatorBacellar, Maria Olívia Amado Ramos-
dc.creatorScott, Phillip-
dc.creatorCarvalho Filho, Edgar Marcelino de-
dc.date.accessioned2014-09-09T15:19:34Z-
dc.date.available2014-09-09T15:19:34Z-
dc.date.issued2013-
dc.identifier.issn1471-2334-
dc.identifier.urihttp://repositorio.ufba.br/ri/handle/ri/15925-
dc.descriptionp. 1-8pt_BR
dc.description.abstractBackground: The main clinical forms of tegumentary leishmaniasis are cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML). L.braziliensis infection is characterized by an exaggerated production of IFN-gamma and TNF-alpha, cytokines involved in parasite destruction, but also in the pathology. Maintenance of an antigen-specific immune response may be important for resistance to re-infection and will contribute for vaccine development. In the present work we investigated the immune response in CL and ML cured individuals. Methods: Participants in the present study included 20 CL and 20 ML patients, who were evaluated prior to, as well as 2 to 15 years after therapy. IFN-gamma, IL-2 and TNF-alpha production were determined by ELISA in supernatants of mononuclear cells stimulated with soluble L.braziliensis antigen (SLA). The frequency of memory CD4+ T cell populations was determined by FACS. Results: Here we show that the majority of CL and ML patients did not produce in vitro IFN-gamma in response to SLA after cure. In the cured individuals who responded to SLA, effector memory (CD45RA-CCR7-) CD4+ T cells were the ones producing IFN-gamma. Because a large percent of CL and ML cured patients lost SLA-induced IFN-gamma production in peripheral blood, we performed Leishmania skin test (LST). A positive LST was found in 87.5% and 100% of CL and ML cured individuals, respectively, who did not produce IFN-gamma or IL-2 in vitro. Conclusion: This study shows that in spite of losing in vitro antigen-specific response to Leishmania, cured CL and ML subjects retain the ability to respond to SLA in vivo. These findings indicate that LST, rather than IFN-gamma production, may be a better assessment of lasting immunity to leishmaniasis in human studies, and thus a better tool for assessing immunization after vaccine. Furthermore, in cured individuals which maintains Leishmania-specific IFN-gamma production, effector memory CD4+ T cells were the main source of this cytokine.pt_BR
dc.language.isoenpt_BR
dc.rightsAcesso Abertopt_BR
dc.sourcehttp://dx.doi.org/10.1186/1471-2334-13-529pt_BR
dc.subjectCured leishmaniasispt_BR
dc.subjectIFN-gammapt_BR
dc.subjectEffector memory CD4+ T cellspt_BR
dc.titleImmunologic response and memory T cells in subjects cured of tegumentary leishmaniasispt_BR
dc.title.alternativeBMC Infectious Diseasespt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 13, n. 1pt_BR
Aparece nas coleções:Artigo Publicado em Periódico (EMV)

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