https://repositorio.ufba.br/handle/ri/14847
Tipo: | Artigo de Periódico |
Título: | Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy |
Título(s) alternativo(s): | FASEB Journal |
Autor(es): | Vasconcelos, Juliana Fraga Souza, Bruno Solano de Freitas Lins, Thayse F. S. Garcia, Letícia M. S. Kaneto, Carla Martins Sampaio, Geraldo P. Alcântara, Adriano Costa de Alcântara, Adriano Costa de Meira, Cássio S. Macambira, Simone Garcia Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
Autor(es): | Vasconcelos, Juliana Fraga Souza, Bruno Solano de Freitas Lins, Thayse F. S. Garcia, Letícia M. S. Kaneto, Carla Martins Sampaio, Geraldo P. Alcântara, Adriano Costa de Alcântara, Adriano Costa de Meira, Cássio S. Macambira, Simone Garcia Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
Abstract: | Chagas disease, caused by Trypanosoma cruzi infection, is a leading cause of heart failure in Latin American countries. In a previous study, we showed beneficial effects of granulocyte colony-stimulating factor (G-CSF) administration in the heart function of mice with chronic T. cruzi infection. Presently, we investigated the mechanisms by which this cytokine exerts its beneficial effects. Mice chronically infected with T. cruzi were treated with human recombinant G-CSF (3 courses of 200 μg/kg/d for 5 d). Inflammation and fibrosis were reduced in the hearts of G-CSF-treated mice, compared with the hearts of vehicle-treated mice, which correlated with decreased syndecan-4, intercellular adhesion molecule-1, and galectin-3 expressions. Marked reductions in interferon-γ and tumor necrosis factor-α and increased interleukin-10 and transforming growth factor-β were found after G-CSF administration. Because the therapy did not induce a Th1 to Th2 immune response deviation, we investigated the role of regulatory T (Treg) cells. A significant increase in CD3+Foxp3+ cells was observed in the hearts of G-CSF-treated mice. In addition, a reduction of parasitism was observed after G-CSF treatment. Our results indicate a role of Treg cells in the immunosuppression induced by G-CSF treatment and reinforces its potential therapeutic use for patients with Chagas disease.—Vasconcelos, J. F., Souza, B. S. F., Lins, T. F. S., Garcia, L. M. S., Kaneto, C. M., Smapaio, G. P., de Alcântara, A. C., Meira, C. S., Macambira, S. G., Ribeiro-dos-Santos, R., Soares, M. B. P. Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy. |
Palavras-chave: | Trypanosoma cruzi Cytokine therapy Inflammation Fibrosis Th1 modulation |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14847 |
Data do documento: | 2013 |
Aparece nas coleções: | Artigo Publicado em Periódico (EMV) |
Arquivo | Descrição | Tamanho | Formato | |
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10.1096fj.13-229351.pdf | 1 MB | Adobe PDF | Visualizar/Abrir |
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