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https://repositorio.ufba.br/handle/ri/14556
Tipo: | Artigo de Periódico |
Título: | Determination of aldehydes in fish by high-performance liquid chromatography |
Título(s) alternativo(s): | Journal of Chromatographic Science |
Autor(es): | Veloso, Márcia Cristina da Cunha Silva, Vilma Mota da Santos, Gislaine Vieira dos Andrade, Jailson Bittencourt de |
Autor(es): | Veloso, Márcia Cristina da Cunha Silva, Vilma Mota da Santos, Gislaine Vieira dos Andrade, Jailson Bittencourt de |
Abstract: | A new analytical method to determine trace volatile aldehydes isolated from the headspace of fish meat at room temperature by high-performance liquid chromatography (HPLC) in the form of 2,4- dinitrophenylhydrazone (DNPHo) derivatives has been developed. Aliquots (50 g) of the fish purée were introduced into a 500-mL glass recipient and were purged with N2 for 40 min through two SEP-PAK C18 cartridges (connected in series) coated with an acid solution of 2,4-dinitrophenylhydrazine. The cartridges were then eluted with acetonitrile (2 mL) and the 2,4-DNPHo formed was quantitated by HPLC-UV analysis using a Zorbax C18 column. The isolated compounds from the dynamic headspace sampling of four kinds of fish species were saturated aldehydes, formaldehyde, acetaldehyde, propanal, butanal, pentanal, and hexanal. Under optimized conditions the detection limits of the HPLC method were in the range of 0.75 nmol/g (formaldehyde) to 2.19 nmol/g (hexanal). The calibration curves were linear in the concentration range from 1.3 nmol/mL to 12.5 nmol/mL. Propanal and acetaldehyde were the major carbonyl compounds identified (ranging from 3.9 nmol/g and 10 nmol/g). This study has revealed the widespread occurrence of formaldehyde, acetaldehyde, propanal, butanal, pentanal, and hexanal in fish meat. |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/14556 |
Data do documento: | 2001 |
Aparece nas coleções: | Artigo Publicado em Periódico (Química) |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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Márcia Cristina da Cunha Veloso.pdf | 142,38 kB | Adobe PDF | Visualizar/Abrir |
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