Determination of carbonyl compounds in the atmosphere of charcoal plants by HPLC and UV detection

Abstract

A chromatographic quantification method with two different mobile phases (elution conditions 1 and 2) was developed to determine carbonyl compounds (CCs) in air samples collected from charcoal production workplaces, using C18 cartridges coated with 2,4-dinitrophenylhydrazine (DNPHi). Several 2,4-dinitrophenylhydrazones (DNPHo) were separated and quantified using an HPLC system and UV detection. In 16 min, elution condition 1 successfully separated and quantified the DNPHo of 14 CC including acetaldehyde, acrolein, formaldehyde, and furfural, and estimated the sum of C4 isomers, butanal–isobutanal–butanone. This elution condition was able to resolve the pairs acrolein/furfural and propanone/propanal, which have been cited in the literature as difficult mixtures to be separated. The elution condition 2 allowed separation and quantification, in less than 30 min, of 13 out of the 17 CC listed above. This elution condition was also able to separate propanone from propanal and butanone from the other components of the C4 mixture. When the two mobile phases were used together, they allowed confirmation of the presence of the DNPHo in the real samples. Thus, both elution conditions have been shown to be appropriate to determine CC, in personal and stationary samples, collected in charcoal production plants.