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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/13902
Tipo: Artigo de Periódico
Título: Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
Título(s) alternativo(s): Clinical and Vaccine Immunology
Autor(es): Mehta, Sanjay R.
Huang, Robert
Yang, Meng
Xing, Quan Zhang
Kolli, Bala
Kwang, Poo Chang
Badaró, Roberto José da Silva
Autor(es): Mehta, Sanjay R.
Huang, Robert
Yang, Meng
Xing, Quan Zhang
Kolli, Bala
Kwang, Poo Chang
Badaró, Roberto José da Silva
Abstract: Leishmania species are obligate intracellular protozoan parasites that cause a broad spectrum of clinical diseases in mammalian hosts. The most frequently used approach to quantify parasites in murine model systems is based on thickness measurements of the footpad or ear after experimental infection. To overcome the limitations of this method, we used a Leishmania mutant episomally transfected with enhanced green fluorescent protein, enabling in vivo real-time whole-body fluorescence imaging, to follow the progression of Leishmania infection in parasitized tissues. Fluorescence correlated with the number of Leishmania parasites in the tissue and demonstrated the real-time efficacy of a therapeutic vaccine. This approach provides several substantial advantages over currently available animal model systems for the in vivo study of immunopathogenesis, prevention, and therapy of leishmaniasis. These include improvements in sensitivity and the ability to acquire real-time data on progression and spread of the infection.
Tipo de Acesso: Acesso Aberto
URI: http://repositorio.ufba.br/ri/handle/ri/13902
Data do documento: 2008
Aparece nas coleções:Artigo Publicado em Periódico (Faculdade de Medicina)

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