https://repositorio.ufba.br/handle/ri/13707
Tipo: | Artigo de Periódico |
Título: | Genetic variability of human immunodeficiency virus-1 in Bahia state, Northeast, Brazil: High diversity of HIV genotypes |
Título(s) alternativo(s): | Journal of Medical Virology |
Autor(es): | Monteiro, Joana Paixão Alcântara, Luiz Carlos Júnior Oliveira, Tulio de Oliveira, Antonio Marcos Melo, Marco Antônio Gomes Brites, Carlos Castro, Bernardo Galvão |
Autor(es): | Monteiro, Joana Paixão Alcântara, Luiz Carlos Júnior Oliveira, Tulio de Oliveira, Antonio Marcos Melo, Marco Antônio Gomes Brites, Carlos Castro, Bernardo Galvão |
Abstract: | The HIV-1 genetic variability in Bahia state, Brazil, was investigated. DNA samples from 229 and 213 HIV-1-infected individuals were analyzed using the heteroduplex mobility assay (HMA) in gag and env fragments, respectively. One hundred seventy-five samples were characterized in both genes. Thirty-two subtype F and BF recombinant viruses were sequenced and analyzed by phylogenetic methods. The combination of HMA and sequencing results showed that seven different HIV-1 genotypes comprised this sample: 147 (84%) B/B, 4 (2.3%) F/F, 3 (1.7%) B/F, 1 (0.6%) F/B, 1 (0.6%) F/D, 1 (0.6%) BF/F, and 18 (10.3%) BF/B. A significant divergence was observed between these two techniques results (84.4%). This is explained by the low accuracy of the HMA for detecting recombinant viruses. These recombinants were unrelated to CRF12, while two sequences were related to CRF28 and CRF29. Nineteen BF mosaics shared the same gag breakpoint. In conclusion, the use of HMA may be inappropriate in regions where different subtypes are co-circulating. Subtype B is the most common genotype, however, an increased prevalence (13.1%) of different BF variants and a potentially new CRF suggest that recombination is occurring frequently in Bahia. These viruses were associated with women infected heterosexually. Finally, this study identified the presence of an F/D recombinant HIV-1 in Brazil. |
Palavras-chave: | HIV-1 Epidemiology Phylogeny Brazil Bahia |
Tipo de Acesso: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/13707 |
Data do documento: | 2009 |
Aparece nas coleções: | Artigo Publicado em Periódico (Faculdade de Medicina) |
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