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metadata.dc.type: Artigo de Periódico
Title: Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
Other Titles: Archives of Oral Biology
Authors: Cury, Patricia Ramos
Carmo, J. P.
Horewicz, V. V.
Santos, Jean Nunes dos
Barbuto, J. A.
metadata.dc.creator: Cury, Patricia Ramos
Carmo, J. P.
Horewicz, V. V.
Santos, Jean Nunes dos
Barbuto, J. A.
Abstract: Objective To investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-MDDCs) derived from individuals with chronic periodontitis (CP) or healthy periodontal tissue (HP). Design m-MDDCs derived from peripheral blood monocytes, cultured for 7 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF), were stimulated with lysates of Streptococcus sanguinis, Prevotella intermedia, Porphyromonas gingivalis, or Treponema denticola on day 4, and were then phenotyped. IL-10, IL-12 and IFN-gamma concentration in the supernatant of cultures were measured. Results Expression of HLA-DR was lower in bacterial-unstimulated mature m-MDDC from CP compared to HP (p = 0.04), while expression of CD1a and CD123 were higher in CP. The expression pattern of HLA-DR, CD11c, CD123, and CD1a did not change on bacterial stimulation, regardless of the bacteria. Stimulation with P. intermedia upregulated CD80 and CD86 in CP cells (p ≤ 0.05). Production of IL-12p70 by bacterial-unstimulated m-MDDCs was 5.8-fold greater in CP compared to HP. Bacterial stimulation further increased IL-12p70 production while decreasing IL-10. Significantly more IFN-gamma was produced in co-cultures of CP m-MDDCs than with HP m-MDDCs when cells were stimulated with P. intermedia (p = 0.009). Conclusions Bacterial-unstimulated m-MDDC from CP exhibited a more immature phenotype but a cytokine profile biased towards proinflammatory response; this pattern was maintained/exacerbated after bacterial stimulation. P. intermedia upregulated co-stimulatory molecules and IFN-gamma expression in CP m-MDDC. These events might contribute to periodontitis pathogenesis.
Keywords: Immune response
Periodontal disease
Periodontal bacteria
Publisher: Archives of Oral Biology
metadata.dc.rights: Acesso Aberto
Issue Date: 2013
Appears in Collections:Artigo Publicado em Periódico (Faculdade de Odontologia)

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