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Use este identificador para citar ou linkar para este item: https://repositorio.ufba.br/handle/ri/13354
Tipo: Artigo de Periódico
Título: Analysis of schistosoma mansoni population structure using total fecal egg sampling
Título(s) alternativo(s): Journal of Parasitology
Autor(es): Blank, Walter A.
Reis, Eliana A. G.
Thiong'o, Fred W.
Braghiroli, João Freitas Melro
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano K.
Carmo, Theomira Mauadie Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
Autor(es): Blank, Walter A.
Reis, Eliana A. G.
Thiong'o, Fred W.
Braghiroli, João Freitas Melro
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano K.
Carmo, Theomira Mauadie Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
Abstract: Many parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias.
URI: http://repositorio.ufba.br/ri/handle/ri/13354
Data do documento: 2009
Aparece nas coleções:Artigo Publicado em Periódico (Faculdade de Medicina)

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