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dc.contributor.authorSouza, Catiane S.-
dc.contributor.authorOliveira, Bruno M.-
dc.contributor.authorCosta, Gustavo G. L.-
dc.contributor.authorSchriefer, Nicolaus Albert Borges-
dc.contributor.authorSchnadelbach, Alessandra Selbach-
dc.contributor.authorUetanabaro, Ana Paula T.-
dc.contributor.authorPirovani, Carlos P.-
dc.creatorSouza, Catiane S.-
dc.creatorOliveira, Bruno M.-
dc.creatorCosta, Gustavo G. L.-
dc.creatorSchriefer, Nicolaus Albert Borges-
dc.creatorSchnadelbach, Alessandra Selbach-
dc.creatorUetanabaro, Ana Paula T.-
dc.creatorPirovani, Carlos P.-
dc.descriptionTexto completo: acesso restrito. p.431-440pt_BR
dc.description.abstractChitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transcription product, and its putative protein in basidioma and secondary mycelium stages of M. perniciosa, a basidiomycotan fungus that causes witches’ broom disease of cacao.pt_BR
dc.subjectCell wallpt_BR
dc.subjectChitin synthasept_BR
dc.subjectWitches’ broom diseasept_BR
dc.titleIdentification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacaopt_BR
dc.title.alternativeJournal of Microbiologypt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.numberv. 47, n. 4pt_BR
Appears in Collections:Artigo Publicado em Periódico (Biologia)

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