Campo DC | Valor | Idioma |
dc.contributor.author | Assis, Ápio Cláudio de Lima | - |
dc.contributor.author | Gonçalves, Islania Giselia Albuquerque | - |
dc.contributor.author | Lima, Renata P. C. | - |
dc.contributor.author | Almeida, Mônica M. | - |
dc.contributor.author | Marinho, Alexsandro Fernandes | - |
dc.contributor.author | Barbosa Filho, José Maria | - |
dc.contributor.author | Cruz, Jader dos Santos | - |
dc.contributor.author | Vasconcelos, Darizy Flavia Silva Amorim de | - |
dc.contributor.author | Medeiros, Isac Almeida de | - |
dc.creator | Assis, Ápio Cláudio de Lima | - |
dc.creator | Gonçalves, Islania Giselia Albuquerque | - |
dc.creator | Lima, Renata P. C. | - |
dc.creator | Almeida, Mônica M. | - |
dc.creator | Marinho, Alexsandro Fernandes | - |
dc.creator | Barbosa Filho, José Maria | - |
dc.creator | Cruz, Jader dos Santos | - |
dc.creator | Vasconcelos, Darizy Flavia Silva Amorim de | - |
dc.creator | Medeiros, Isac Almeida de | - |
dc.date.accessioned | 2013-07-11T13:29:29Z | - |
dc.date.available | 2013-07-11T13:29:29Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0305-1870 | - |
dc.identifier.uri | http://www.repositorio.ufba.br/ri/handle/ri/12166 | - |
dc.description | Texto completo. Acesso restrito. p. 37-44 | pt_BR |
dc.description.abstract | The present study used functional and electrophysiological
approaches to investigate the mechanisms by which warifteine,
a bisbenzylisoquinoline alkaloid isolated from Cissampelos sympodialis
Eichl., causes vasorelaxation of the rat thoracic aorta.
2. Warifteine (1 pmol/L–10 lmol/L) induced concentration-
dependent relaxation (pD2 = 9.40 ± 0.06; n = 5) of
endothelium-intact aortic rings precontracted with noradrenaline
(10–100 lmol/L). The relaxation effects were
not attenuated by removal of the endothelium. Warifteine
also induced the relaxation of prostaglandin F2a
(1–10 mmol/L)-precontracted rings (pD2 = 9.2 ± 0.2;
n = 8). In contrast, the relaxant activity of warifteine was
nearly abolished in high K+ (80 mmol/L)-precontracted
aortic rings. In preparations incubated with 20 mmol/L
KCl or with the K+ channel blockers tetraethylammonium
(1, 3 and 5 mmol/L), iberiotoxin (20 nmol/L), 4-aminopyridine
(1 mmol/L) or glibenclamide (10 lmol/L), the
vasorelaxant activity of warifteine was markedly reduced.
However, BaCl2 (1 mmol/L) had no effect on the relaxant
effects of warifteine.
3. In vascular myocytes, warifteine (100 nmol/L) significantly
increased whole-cell K+ currents (at 70 mV). Under
nominally Ca2+-free conditions, warifteine did not reduce
extracellular Ca2+-induced contractions in rings precontracted
with high K+ or noradrenaline (100 lmol/L).
4. Together, the results of the present study indicate that
warifteine induces potent concentration-dependent relaxation
in the rat aorta via an endothelium-independent mechanism
that involves the activation of K+ channels. | pt_BR |
dc.language.iso | en | pt_BR |
dc.publisher | Clinical and Experimental Pharmacology and Physiology | pt_BR |
dc.source | 10.1111/1440-1681.12029 | pt_BR |
dc.subject | bisbenzylisoquinoline alkaloid | pt_BR |
dc.subject | potassium channels | pt_BR |
dc.subject | rat aorta | pt_BR |
dc.subject | vascular smooth muscle cells | pt_BR |
dc.subject | vasodilatation | pt_BR |
dc.subject | warifteine | pt_BR |
dc.title | Warifteine, a bisbenzylisoquinoline alkaloid, induces relaxation by activating potassium channels in vascular myocytes | pt_BR |
dc.title.alternative | Clinical and Experimental Pharmacology and Physiology | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.description.localpub | Salvador | pt_BR |
dc.identifier.number | v. 40, n. 1 | pt_BR |
Aparece nas coleções: | Artigo Publicado em Periódico (Biologia)
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