Please use this identifier to cite or link to this item:
https://repositorio.ufba.br/handle/ri/17698
metadata.dc.type: | Artigo de Periódico |
Title: | A proteomic approach to identify proteins from Trichuris trichiura extract with immunomodulatory effects |
Authors: | Santos, L. N. Gallo, M. B. Silva, E. S. Figueiredo, C. A. Cooper, P. J. Barreto, Mauricio Lima Loureiro, Sebastião Carvalho, Lain Carlos Pontes de Alcântara-Neves, Neuza Maria |
metadata.dc.creator: | Santos, L. N. Gallo, M. B. Silva, E. S. Figueiredo, C. A. Cooper, P. J. Barreto, Mauricio Lima Loureiro, Sebastião Carvalho, Lain Carlos Pontes de Alcântara-Neves, Neuza Maria |
Abstract: | Infections with Trichuris trichiura and other trichurid nematodes have been reported to display protective effects against atopy, allergic and autoimmune diseases. The aims of the present study were to investigate the immunomodulatory properties of T. trichiura adult worm extract (TtE) and its fractions (TtEFs) on the production of cytokines by peripheral blood mononuclear cells and to identify their proteinaceous components. Fourteen TtEFs were obtained by ion exchange chromatography and tested for effects on cytokine production by peripheral blood mononuclear cells. The molecular constituents of the six most active fractions were evaluated using nano-LC/mass spectrometry. The homology between T. trichiura and the related nematode Trichinella spiralis was used to identify 12 proteins in TtEFs. Among those identified, fructose biphosphate aldolase, a homologue of macrophage migration inhibitory factor and heat-shock protein 70 may contribute to the immunomodulatory effects of TtEFs. The identification of such proteins could lead to the development of novel drugs for the therapy of allergic and other inflammatory diseases. |
Keywords: | Cytokine Immunoregulation Mononuclear Mells Proteomics Trichuris spp |
metadata.dc.rights: | Acesso Aberto |
URI: | http://repositorio.ufba.br/ri/handle/ri/17698 |
Issue Date: | Jun-2013 |
Appears in Collections: | Artigo Publicado em Periódico Estrangeiro (ISC) |
Files in This Item:
File | Description | Size | Format | |
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Barreto 2013.pdf | 1,57 MB | Adobe PDF | View/Open |
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