Por favor, use este identificador para citar o enlazar este ítem:
https://repositorio.ufba.br/handle/ri/13354
metadata.dc.type: | Artigo de Periódico |
Título : | Analysis of schistosoma mansoni population structure using total fecal egg sampling |
Otros títulos : | Journal of Parasitology |
Autor : | Blank, Walter A. Reis, Eliana A. G. Thiong'o, Fred W. Braghiroli, João Freitas Melro Santos, Jarbas M. Melo, Paulo Roberto Santana de Guimarães, Isabel C. S. Silva, Luciano K. Carmo, Theomira Mauadie Azevedo Reis, Mitermayer Galvão dos Blanton, Ronald Edward |
metadata.dc.creator: | Blank, Walter A. Reis, Eliana A. G. Thiong'o, Fred W. Braghiroli, João Freitas Melro Santos, Jarbas M. Melo, Paulo Roberto Santana de Guimarães, Isabel C. S. Silva, Luciano K. Carmo, Theomira Mauadie Azevedo Reis, Mitermayer Galvão dos Blanton, Ronald Edward |
Resumen : | Many parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias. |
URI : | http://repositorio.ufba.br/ri/handle/ri/13354 |
Fecha de publicación : | 2009 |
Aparece en las colecciones: | Artigo Publicado em Periódico (Faculdade de Medicina) |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
10.1645-GE-1895.1.pdf | 2,05 MB | Adobe PDF | Visualizar/Abrir |
Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.