Brito, Rafaela Carvalhais; 0614611431171886; http://lattes.cnpq.br/0614611431171886
Resumo:
Snakebite envenoming is considered a public health problem in the world and a
neglected tropical disease by the World Health Organization due to the impacts on
the population and health systems. This present study aimed to obtain hyperimmune
serum anti-crotalic produced in sheep and to evaluate the humoral immune
response, throughout the immunization schedule. In addition, the efficacy of this
serotherapy in neutralizing the lethality activity of Crotalus spp., in mice was
analyzed. The immunization of sheep occurred according to the schedule of the
Standard Operational Protocol of the Vital Brazil Institute for the production of
anticrotalic serum, also observing the occurrence of local changes and clinical signs
in these animals. Serum IgG levels were quantified and evaluated for the recognition
specificity of the various venom constituents before, during and after the end of the
hyperimmunization process to evaluate their production kinetics. The protocol used,
the seroproducer animal choicen and doses utilized were effective in obtaining a
strong immune response with high antibodies levels and high specificity against the
venom of Crotalus spp., inhibiting 100% lethality activity by preincubation with
venom. Thus, the present study demonstrated that the production of anticrotalic
antivenom using regionalized raw material is needed to neutralize the main
components of this venom, mitigating the occurrence of permanent sequel and
deaths by the rattlesnakes bite on the State of Bahia.
