Avaliação do potencial alergênico do ácaro de poeira doméstica Glycycometus malaysiensis

Abstract

Introduction: Dust mites are considered one of the main sources of aeroallergens and are associated with the development of allergic diseases. In Brazil, the most prevalent mites belong to the genera Dermatophagoides spp and Blomia spp. However, in a previous study conducted by our group we identified for the first time the presence of the mite Glycycometus malaysiensis, which had already been reported as a sensitizing agent in Asian countries. Both G. malaysiensis and B. tropicalis mites exhibit morphological similarities, raising the possibility of cross-reactivity between their allergens. Nevertheless, specific allergens of this mite species have not been fully characterized. Objectives: Identific and isolate of the mite G.malaysiensis from house dust and evaluate of antigen reactivity in serum from atopic patients. Material and Methods: The allergenic extracts were identified using the multiplex PCR technique and characterized using SDS-PAGE and Western Blot. A total of 57 sera from atopic patients had IgE reactivity to these extracts assessed by indirect ELISA. Results: Using Western blots without inhibition to the extract of D. pteronyssinus, was observed a positive reactivity of the atopic patients to the bands referring to the G. malaysiensis mite. In addition, the patients tested showed greater IgE reactivity to the extracts identified with the D. pteronyssinus and G. malaysiensis mites compared to the extract containing only the D. pteronyssinus mite. Conclusions: In this study, we could observe the immunogenic potential of G. malaysiensis and its possible significance in the evaluated indiviudals.