Avaliação da expressão gênica de osteoblastos cultivados em biomateriais (pla, pc-iso, abs m30i)

Abstract

Some orofacial injuries are related to the loss of bone continuity and, consequently, to structural and functional damage that impair quality of life. The production of biological tissues from cell cultures is suggested as an auxiliary strategy for orofacial reconstructive surgeries, but the effectiveness of the technique depends on a support structure that guides the formation of functional tissues according to the receptor bed. In this context, the gene expression signature of osteoblastic cells (SAOS-2) cultured on 5 and 10 days in rigid matrices produced with a three-dimensional printer was investigated. To this end, osteoblasts cultured on polylactic acid polycarbonate (PLA), acrylonitrile-butadiene-styrene (ABS M30i) and Polycarbonate-ISO (PC-ISO) scaffolds were evaluated by Array Real time PCR system to determine the expression of genes related to osteoblastic differentiation, bone mineralization, ossification, growth factors, and transcription factors involved in inflammation and healing. The results obtained showed that the ABS-M30i scaffolds, with emphasis on the genes bmp5, bmp6, col9A2, col11A1, spp1, Ibsp, fgf2, vegfA, smad4, smad9, runx2 / bmpr1A, fgfr1 and Igfr1, the PC-ISO with emphasis on in the genes bmp5, bmp6, col19A1, col11A1, cfs2, Ibsp, fgf2, vegfa, smad1, runx2, bmPr1a and Fgfr and the PLA, highlighting the genes bmp5, bmp6, col19A1, col11A1, cfs2, spp1, minpp1, ibsp, fgf2 , tgfb2, smad1, smad5, smad4, bmpr1 and fgr1, allow the expression of a large number of genes responsible for bone formation in vitro, however, PLA seems to have allowed more exuberant expression in the genes that were highlighted, and PC-ISO allowed a more uniform overall response. Thus, the use of the three biomaterials in tissue engineering becomes promising.