Avaliação do potencial de Pseudomonas sp. aplicada à recuperação avançada de petróleo

Abstract

The purpose of this work was to evaluate the ability of the bacterium Pseudomonas sp. in the biotransformation of alkanes in order to propose sustainable solutions that can be applied in advanced oil recovery, in addition to determining strain growth patterns under extreme conditions. For this, the work was carried out under laboratory conditions, initially, with the fractionation of crude oil to obtain the saturated fraction, used in the experiment. The bacteria tolerance test to salinity and temperature was also carried out to determine the conditions for setting up the experiment in relation to these parameters. Additionally, an experiment was set up to produce biosurfactant, through biostimulation. The biotransformation experiment consisted of a triplicate with treatment and a control. For treatments, erlenmeyrs received 100 mL of broth containing the biosurfactant, 10 g (10%) of NaCl, 3% of the strain and 1% of the saturated fraction. Erlenmeyrs were incubated at 40 ºC and 180 rpm for 18 days with periodic analysis. As results were initially observed the tolerance of bacteria that had better performance for the temperature of 40º C and there was no significant change for the different salinities, being a non-limiting parameter. For the final experiment the bacterial growth analyzed by O.D. had a low variation with the lowest point in T18 with absorbance of 0.115 and the highest point in T6 with absorbance of 0.149. For the analysis of the bacterial population through qPCR, the pattern found is similar to the optical density results, with low variation, with the lowest number of copies of the 16S rRNA gene 6.66x 103 being found in T0 and the highest number was in T12 with number of copies 7.86x 103 . For biotransformation analysis, time 6 was observed with the highest rate, being 54% of oil recovery (C30), followed by 52% (C31) and 51% (C29).