Caracterização de propriedades físicas e celulares de membranas de L-PRFs produzidas por diferentes centrífugas/ protocolos de centrifugação

Abstract

Leukocyte and Platelet-Rich Fibrin (L-PRF) has been used as a regulator of the healing process because it is an important source of cytokines and growth factors. L-PRF is an autologous product from the centrifugation of blood samples. The success of the technique is intrinsically related to the blood processing protocols collected and the type of used centrifuge. This study aimed to evaluate, comparatively macro and microscopically using the hematoxylin/eosin (H/E) technique, the formation of the L-PRF membrane/clot in two different models of centrifuges, the Intra-Spin - Intra-Lock and the Montserrat centrifuge. Four blood samples were used from 8 healthy volunteers who underwent rehabilitation surgery with dental implants. Two types of centrifuge with different centrifugation protocols were used. In group 1 of the Montserrat centrifuge, 400gm of centrifugal force at 10 minutes, 27ºC and 2700 rpm were used, while in group 2 of the IntraSpin Intra-Lock centrifuge, 400g of centrifugal force at 12 minutes, 27ºC and 2700 rpm were used. The blood was collected quickly and randomly distributed in the centrifuges. At the end, 32 clot/membrane L-PRFs were obtained. Then, the pieces were registered and sent for macroscopic analysis, of the variables of weights and sizes of clots/membranes of L-PRFs and weight of the supernatant/clot of red blood cells remaining in the tubes. Then, the specimens were processed for routine histological technique (H/E). Each slide was divided into three areas of equal size: Proximal (Head and Face), Center (Body) and Distal (Tail), andw was analyzed semiquantitatively for the presence of leukocytes. The results demonstrate that the membranes produced in the IntraSpin - Intra-Lock centrifuge present leukocytes in, at least, 2 areas simultaneously (62.5%) (p = 0.003). Regarding leukocyte intensity (p = 0.012), leukocyte percentage (p = 0.037), leukocyte distribution (p = 0.005) and leukocyte location (p = 0.037), both were statistically higher in the central areas of the LPRFs produced in the Intra-Spin - Intra-Lock centrifuge when compared to the central areas of those produced in Montserrat. With regard to macroscopic characteristics, no statistically relevant differences were found in any of the LPRFs produced by the two centrifuges. It is suggested that the different protocols influence the presence and distribution of leukocytes between the membranes of L-PRFs. New studies must be carried out to expand this characterization.