https://repositorio.ufba.br/handle/ri/23449 2026-04-17T18:11:40Z 2026-04-17T18:11:40Z Meira, Paloma R. David, Juceni P. Ribeiro, Erika M. de O. Santana, José R.F. Brandão, Hugo N. Oliveira, Lenaldo M. de David, Jorge M. Medrado, Héctor H. Pastore, José F.B. https://repositorio.ufba.br/handle/ri/26003 2022-10-21T22:37:47Z 2017-01-01T00:00:00Z

Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro Meira, Paloma R.; David, Juceni P.; Ribeiro, Erika M. de O.; Santana, José R.F.; Brandão, Hugo N.; Oliveira, Lenaldo M. de; David, Jorge M.; Medrado, Héctor H.; Pastore, José F.B. This work describes an in vitro propagation protocol for the large-scale cultivation of Leptohyptis macrostachys (Benth.) Harley & JFB Pastore and the influence of abiotic factors on podophyllotoxin and yatein production. The plant was established from seeds collected in Chapada Diamantina, BA, Brazil and submitted to different growth mediums and physical conditions. The podophyllotoxin and yatein contents were quantified by HPLC/DAD and with pure standards and these two lignans were present in all experiments. The lignan quantities were evaluated using the Sisvar Program, compared by Tukey’s test and hierarchical cluster analysis and principal component analysis. In all experiments, podophyllotoxin and yatein were detected at different concentrations. The best protocol cultivar of L. macrostachys was established from seeds in MS ½ medium supplemented with 1.5% sucrose and 11.55 μM of gibberellic acid A3 (GA3) at 30 °C, which yielded the highest concentration of podophyllotoxin (5.831 mg g−1). These results are important findings for the production of podophyllotoxin from the tissue culture. Artigo de Periódico

2017-01-01T00:00:00Z Esteves, Lucas Senhorinho Heriques, Águida Cristina Gomes Silva, Carolina Avila Varginha de Moraes e Cangussu, Maria Cristina Teixeira Ramos, Eduardo Antônio Gonçalves Estrela, Carlos Santos, Jean Nunes dos https://repositorio.ufba.br/handle/ri/25934 2022-08-26T14:02:51Z 2017-01-01T00:00:00Z

Actinomycosis is not Frequent in the Periapex But is a Persistent Lesion Esteves, Lucas Senhorinho; Heriques, Águida Cristina Gomes; Silva, Carolina Avila Varginha de Moraes e; Cangussu, Maria Cristina Teixeira; Ramos, Eduardo Antônio Gonçalves; Estrela, Carlos; Santos, Jean Nunes dos Periapical actinomycosis caused by a gram-positive anaerobic pathogen characterizes a typical extra-radicular infection. This study determined the frequency and correlated the content of bacteria colonies with the of periapical actinomycosis size. The study comprised a total of 218 periapical lesions (PL) (cysts, granulomas or abscess). The specimens embedded in paraffin were sliced into 4-μm sections and stained with hematoxylin-eosin, Gram, Periodic Acid–Schiff (PAS) and Grocott’s stain. The presence of bacterial colonies composed of filamentous structures labeled with the histochemical stains were described as Actinomyces, and for each case, the bacterial colonies were counted and measured. The correlation between the number and size of bacterial colonies and the size of PL was tested using Pearson’s adjusted correlation coefficient. From 218 PL, bacterial colonies were identified in 64 biopsies. Seven cases (0.3%) fulfill the criteria for diagnosis of periapical actinomycosis. All of cases were therapy-resistant and did not showed periapical repair after 12 months of follow-up. Periapical surgery or dental extraction was performed. The correlation test indicated no correlation between the number of bacterial colonies and the lesion size (p=0.752, r=-0.148). However, a larger bacterial colony size generally resulted in a larger periapical lesion (P=0.000, r=0.657). The frequency of periapical actinomycosis was low, and this lesion should be included in the differential diagnosis of PL. The size of the Actinomyces colonies seemed to contribute to increase the size of the periapical lesion. Artigo de Periódico

2017-01-01T00:00:00Z Gomes, Silvana Vieira Floresta Portugal, Lindomar Andrade Anjos, Jeancarlo Pereira dos Jesus, Onildo Nunes de Oliveira, Eder Jorge de David, Juceni Pereira de Lima David, Jorge Mauricio https://repositorio.ufba.br/handle/ri/25933 2022-09-21T17:46:06Z 2017-01-01T00:00:00Z

Accelerated solvent extraction of phenolic compounds exploiting a Box-Behnken design and quantification of five flavonoids by HPLC-DAD in Passiflora species Gomes, Silvana Vieira Floresta; Portugal, Lindomar Andrade; Anjos, Jeancarlo Pereira dos; Jesus, Onildo Nunes de; Oliveira, Eder Jorge de; David, Juceni Pereira de Lima; David, Jorge Mauricio This work describes the development of a method for the extraction of phenolic compounds, mainly flavonoids, from species of Passiflora by employing accelerated solvent extraction (ASE) and using a Box-Behnken design with desirability functions for optimization. The optimal extraction conditions consisted of an extraction temperature of 80 °C, 64% (w/w) ethanol and five number of extraction cycles. A high-performance liquid chromatography- diode array detection (HPLC-DAD) method for the identification and quantification of orientin, isoorientin, vitexin, isovitexin and rutin flavonoids in the leaves of seventeen Passiflora spp. using gradient elution with acetonitrile (solvent B) and 0.2% (w/w) formic acid in water (solvent A) as the eluent mixture was also developed and validated. These five flavonoids were quantified with good linearity, LOQs, LODs, precision, and accuracy. Higher concentrations of isoorientin, orientin, vitexin, isovitexin and rutin were determinate in P. edulis f. flavicarpa (1.61 mg/g extract and 0.58 mg/g dry plant), P. morifolia (2.10 mg/g extract and 0.90 mg/g dry plant), P. setacea (2.48 mg/g extract and 0.97 mg/g dry plant), P. setacea (8.46 mg/g extract and 3.30 mg/g dry plant) and P. galbana (3.48 mg/g extract and 1.02 mg/g dry plant), respectively. Artigo de Periódico

2017-01-01T00:00:00Z Silva, Gabriele Marisco da Santos, Refineide Xavier Aguiar, Rosane Moura Brendel, Martin Pungartinik, Cristina https://repositorio.ufba.br/handle/ri/25932 2022-08-26T14:03:37Z 2018-05-04T00:00:00Z

Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao Silva, Gabriele Marisco da; Santos, Refineide Xavier; Aguiar, Rosane Moura; Brendel, Martin; Pungartinik, Cristina The present in vitro study evaluates antifungal properties of Spondias purpurea plant extract against Moniliophthora perniciosa. Fractions of Ethanol Crude Extracts (CE) from S. purpurea were tested for their antifungal activity. CE inhibited mycelial growth of M. perniciosa by 60%at a dose of 10 mg/mL, while survival of broken hyphae of the fungus was inactivated by 90% at a dose of 8 mg/mL. Fractionation of CE yielded a terpene containing sub fraction (FA) with the highest fungicidal activity. FA acted as oxidant and its application induced oxidative stress in M. perniciosa that may be the responsible for the observed induced cell death. The chromatographic profile obtained with FA of S. purpurea revealed a variety of terpenes, which included Spathulenol (14.2%), Linolenic acid (8.4%), trans Caryophyllene (6.9%), and Alpha-muurolene (6.9%).This study describes for the first time effects of terpenes of S. purpurea related to induced oxidative stress in fungus. S. purpurea, therefore, may be a natural source for isolation of antifungal compounds against M. perniciosa. This characteristic may be useful in application as botanical fungicide in biological control. Artigo de Periódico

2018-05-04T00:00:00Z