RI UFBA >
Escola de Nutrição >
Artigos Publicados em Periódicos (Nutrição) >
Please use this identifier to cite or link to this item:
|Title: ||Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment|
|Other Titles: ||Brazilian Journal of Microbiology|
|Authors: ||Oliveira, I. C.|
Almeida, Rogeria Comastri de Castro
Almeida, Paulo Fernando de
|Keywords: ||Listeria;bacteriophage A511;laser light;detection;skimmed milk|
|Issue Date: ||2012|
|Publisher: ||Brazilian Journal of Microbiology|
|Abstract: ||A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable
Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay.
Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of
exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 108 pfu/mL) without
affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean
populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration.
Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously
tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an
alternative for rapid, sensitive and quantitative detection of L. monocytogenes.|
|Description: ||p. 1128-1136|
|Appears in Collections:||Artigos Publicados em Periódicos (Nutrição)|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.