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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/8008

Title: Bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
Other Titles: Brazilian Journal of Microbiology
Authors: Oliveira, I. C.
Almeida, Rogeria Comastri de Castro
Hofer, Ernesto
Almeida, Paulo Fernando de
Keywords: Listeria;bacteriophage A511;laser light;detection;skimmed milk
Issue Date: 2012
Publisher: Brazilian Journal of Microbiology
Abstract: A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 108 pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes.
Description: p. 1128-1136
URI: http://www.repositorio.ufba.br/ri/handle/ri/8008
ISSN: 1517-8382
Appears in Collections:Artigos Publicados em Periódicos (Nutrição)

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