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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/7598

Title: IgG anti-Ige autoantibodies in visceral leishmaniasis
Other Titles: Memórias do Instituto Oswaldo Cruz
Authors: Atta, Ajax Mercês
Atta, Maria Luiza Brito de Sousa
D'Oliveira Junior, Argemiro
Almeida, Roque Pacheco de
Araujo, Maria Ilma Andrade Santos
Carvalho Filho, Edgar Marcelino de
Keywords: IgE;IgG anti-IgE;protein G;visceral leishmaniasis;schistosomiasis mansoni
Issue Date: Jan-2002
Abstract: Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.
Description: p. 101-103
URI: http://www.repositorio.ufba.br/ri/handle/ri/7598
ISSN: 0074-0276
Appears in Collections:Artigos Publicados em Periódicos (FARMACIA)

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