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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/6692

Title: Comparison of three decontamination methods for Mycobacterium bovis isolation
Other Titles: Brazilian Journal of Microbiology
Authors: Ambrosio, Simone Rodriguez
Oliveira, Eugênia Márcia de Deus
Rosales Rodriguez, Cesar Alejandro
Ferreira Neto, José Soares
Amaku, Marcos
Keywords: Mycobacterium bovis;Decontamination;Petroff;HPC
Issue Date: Apr-2008
Publisher: Sociedade Brasileira de Microbiologia
Abstract: Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
Description: p.241-244
URI: http://www.repositorio.ufba.br/ri/handle/ri/6692
ISSN: 1517-8382
Appears in Collections:Artigos Publicados em Periódicos (EMV)

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