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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/5248

Title: Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
Other Titles: Veterinary Immunology and Immunopathology
Authors: Paule, Bruno Jean Adrien
Azevedo, Vasco Ariston de Carvalho
Regis, Lia Fernandes
Bahia, Robson Cerqueira
Carminati, Renato
Vale, Vera Lucia Costa
Costa, Lilia Ferreira Moura
Freire, Songeli Menezes
Schaer, Robert Eduard
Nascimento, Ivana Lúcia de Oliveira
Goes, Alfredo Miranda de
Nascimento, Roberto José Meyer
Keywords: Goats;Corynebacterium pseudotuberculosis;Interferon-g;Serology;Kinetic;Caseous lymphadenitis
Issue Date: 2003
Abstract: Corynebacterium pseudotuberculosis is the cause of caseous lymphadenitis (CLA) in small ruminants, a chronic granulomatous disease that provokes significant zootechnics losses to ovine and goat breeders in northern Brazil. The present work was conducted to analyse aspects of humoral and cellular immune responses after experimental infection. Eight goats were infected intradermally with a single dose of virulent C. pseudotuberculosis strain and specific IgG, interferon-g (IFN-g) production as well as IgG avidity and antigens pattern recognition dynamics against an excreted–secreted antigen were recorded during 20 weeks. At the end of the follow-up, animals were slaughtered and necropsied. Although no animals showed apparent clinical signs of infection at the end of the trial, IFN-g response, even more so than the humoral response, differentiated animals into two groups of high or medium/low response. The time-course of IFN-g production presented a short-lived primary response on day 5 after infection of animals of both groups, and a strong and long lasting secondary response starting on day 16 after infection in the high response group. The indirect ELISA used was able to detect a positive antibody titre between 6 and 11 days after infection in the two groups. IgG avidity index oscillated initially between 15 and 45%, and showed approximately 5% units increment during the 20 follow-up weeks. With only one individual exception, the qualitative antigens pattern recognition showed on day 11 after infection remained constant through the experiment. IgG avidity is highly correlated with IgG production, but could not be related with specific immunodominant bands. Both humoral and cellular responses kinetics presented a similar pattern of activation/deactivation but necropsy results suggested that the IFN-g test would be a very specific marker of CLA status.
Description: p.129–139
URI: http://www.repositorio.ufba.br/ri/handle/ri/5248
ISSN: 0165-2427
Appears in Collections:Artigos Publicados em Periódicos (ICS)

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