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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/20548

Title: Production and use of egg-yolk antibody for detection of canine parvovirus in feces
Other Titles: Revista de Ciências Médicas e Biológicas
Authors: Guimarães, Marco Cesar Cunegündes
Amaral, Livia Gomes
Borges, Franz Viana
Vieira, Hugo Paes Leme
Shimoya, Aldo
Matta, Claudia Gomes Fernandes
Matta, Marcos Fernando de Rezende
Keywords: Parvovírus canino-Diagnóstico- HA-HI.;Anticorpos IgY.;ELISA indireto.
Issue Date: Sep-2008
Publisher: Instituto de Ciências da Saúde/ Universidade Federal da Bahia
Citation: GUIMARÃES, M. C. C. et al. Production and use of egg-yolk antibody for detection of canine parvovirus in feces. R. Ci. méd. biol., Salvador, v.7, n.3, p. 241-248, set./dez. 2008.
Abstract: Canine parvovirus (CPV), cause an intestinal disease characterized by bloody diarrhea, is often fatal in puppies. The virus is transmitted by contact with infected dogs or their feces. The virus is very stable in the environment and may survive for several months in contaminated areas. The CPV attacks the rapidly diving cells of the bone marrow and the small intestine. Several laboratory tests have been developed and are available for specific viral diagnosis. Where facilities are available, rapid diagnosis can be made by electron microscopy (EM) of fecal material from cases with typical signs of disease. The virus also can be isolated in several feline and canine cell lines such as canine and feline kidney cells, but isolation is seldom used in practice since cell cultures are required and at least 1 week for results is required. Fecal hemagglutination-hemagglutination (HA-HI) tests have provided a simple and rapid method for detecting virus in fecal and tissue samples and are employed by several diagnostic laboratories, however the HA test is less sensitive than EM or enzyme-linked immunoassays (ELISA). In this work, the laying chickens are immunized with the canine parvovirus strain Cornell 780916-80 and the egg yolk antibody (IgY) isolated and characterized by indirect ELISA to detect canine parvovirus in feces.
Description: Keywords: Canine parvovirus - Diagnosis - Egg yolk antibody- ELISA, indirect; Canine parvovirus - Diagnosis - HA-HI
URI: http://repositorio.ufba.br/ri/handle/ri/20548
ISSN: 2236-5222
Appears in Collections:Artigos Publicados em Periódicos (PPGORGSISTEM)

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