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|Title: ||Molecular frequency and isolation of cyst-forming coccidia from free ranging chickens in Bahia State, Brazil|
|Other Titles: ||Veterinary Parasitology|
|Authors: ||Gonçalves, Ilka N.|
Uzêda, Rosângela Soares
Lacerda, Geiselane A.
Moreira, Rose R. N.
Araújo, Flábio R.
Oliveira, R. H. M.
Corbellini, Luís Gustavo
Gondim, Luis Fernando Pita
|Keywords: ||Toxoplasma gondii;Neospora caninum;Hammondia spp;PCR;IFAT;Bioassay|
|Issue Date: ||2012|
|Abstract: ||The Toxoplasmatinae parasites Toxoplasma gondii, Neospora caninum and Hammondia spp. have carnivores as definitive hosts that shed the parasite oocysts in their feces. Birds that feed directly from the soil, such as chickens, are exposed to infection and may serve as indicators of the presence of the parasite in the environment and as a source of infection for other animals. The aims of this study were to determine the frequency of infection by these parasites in free ranging chickens, to test whether chickens are intermediate hosts of Hammondia spp., and to isolate N. caninum from chickens. One hundred chickens, which were raised in contact to cattle and dogs, were bought in five towns located in Bahia, Brazil. Blood and tissues (brain and heart) were used for serology, molecular tests and bioassay in mice for parasite isolation. T. gondii DNA was detected in 29 chickens, and N. caninum DNA was observed in six animals. Hammondia spp. DNA was not detected in tissues from any chicken. Tissues from eight N. caninum seropositive chickens were bioassayed in interferon-gamma gene knockout mice, but the mice did not become infected; T. gondii was isolated from six of 14 seropositive chickens after bioassay in outbreed Swiss mice. The authors concluded that: chickens seem to be better hosts for T. gondii when compared to N. caninum, based on the molecular and bioassay results; Hammondia spp. probably does not infect chickens or is rarely found in this animal species.|
|Description: ||Texto completo: acesso restrito. p. 74–79|
|Appears in Collections:||Artigos Publicados em Periódicos (EMV)|
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