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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/15039

Title: Effect of low-level laser therapy (λ660 nm) on angiogenesis in wound healing: a immunohistochemical study in a rodent model
Other Titles: Brazilian Dental Journal
Authors: Colombo, Fábio
Valença Neto, Alberto de Aguiar Pires
Sousa, Ana Paula Cavalcanti de
Marchionni, Antônio Márcio Teixeira
Pinheiro, Antonio Luiz Barbosa
Reis, Silvia Regina de Almeida
Keywords: Angiogenesis;TGF-β;Laser;Repair;Granulation tissue;Mast cells;Vasodilation
Issue Date: 2013
Abstract: The aim of the present investigation was to evaluate the angiogenesis on dorsal cutaneous wounds in a rodent model treated with λ660 nm laser light. New vessel formation is a multistep process involving vessel sprouting, endothelial cell migration, proliferation and tube formation. Although several in vivo studies have shown that laser phototherapy influences tissue repair, a fully understanding of angiogenesis mechanisms are not yet known. Twenty-four young adult male Wistar rats weighing between 200 and 250 g were used. Under general anesthesia, one excisional wound was created on the dorsum of each animal and they were randomly distributed into two groups: one control and one treated with laser (λ660 nm, 16 mW, 10 J/cm2). Each group was subdivided into three subgroups according to the animal death timing (2, 4 and 6 days). Laser irradiation started immediately after surgery and was repeated every other day during the experiment and marked with Sirius Red, specific for collagen, and immunomarked with anti-TGF-β and anti-von Willebrand factor. Marked sections underwent histological analysis by light microscopy and the mean area of the wound of each animal was calculated and analyzed by ANOVA and Tukey's test (α=0.05). Although at some death periods, collagen expression and number of blood vessels on irradiated animals were higher than in the control ones, no significant differences were found at any time in relation to TGF-β expression (p>0.05). It was concluded that laser treatment (λ660 nm) contributed to increase angiogenesis.
Description: p. 308-312
URI: http://repositorio.ufba.br/ri/handle/ri/15039
ISSN: 0103-6440
Appears in Collections:Artigos Publicados em Periódicos (Odontologia)

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