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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/14028

Title: Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis
Other Titles: Journal of Leukocyte Biology
Authors: Afonso, Lilian
Borges, Valéria de Matos
Cruz, Heloísa
Gomes, Flávia L. Ribeiro
Reis, George A. dos
Dutra, Alberto Augusto Noronha
Clarêncio, Jorge
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Keywords: Apoptosis;Necrosis;Phagocytosis;TGF-β1;NE;Leishmaniose
Issue Date: 2008
Abstract: Neutrophils are involved in the initial steps of most responses to pathogens. In the present study, we evaluated the effects of the interaction of apoptotic vs. necrotic human neutrophils on macrophage infection by Leishmania amazonensis. Phagocytosis of apoptotic, but not viable, neutrophils by Leishmania-infected macrophages led to an increase in parasite burden via a mechanism dependent on TGF-β1 and PGE2. Conversely, infected macrophages’ uptake of necrotic neutrophils induced killing of L. amazonensis. Leishmanicidal activity was dependent on TNF-α and neutrophilic elastase. Nitric oxide was not involved in the killing of parasites, but the interaction of necrotic neutrophils with infected macrophages resulted in high superoxide production, a process reversed by catalase, an inhibitor of reactive oxygen intermediate production. Initial events after Leishmania infection involve interactions with neutrophils; we demonstrate that phagocytosis of these cells in an apoptotic or necrotic stage can influence the outcome of infection, driving either parasite survival or destruction.
Description: Texto completo: acesso restrito. p. 389-396
URI: http://repositorio.ufba.br/ri/handle/ri/14028
ISSN: 0741-5400
Appears in Collections:Artigos Publicados em Periódicos (Biologia)

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