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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/13707

Title: Genetic variability of human immunodeficiency virus-1 in Bahia state, Northeast, Brazil: High diversity of HIV genotypes
Other Titles: Journal of Medical Virology
Authors: Monteiro, Joana Paixão
Alcântara, Luiz Carlos Júnior
Oliveira, Tulio de
Oliveira, Antonio Marcos
Melo, Marco Antônio Gomes
Brites, Carlos
Castro, Bernardo Galvão
Keywords: HIV-1;Epidemiology;Phylogeny;Brazil;Bahia
Issue Date: 2009
Abstract: The HIV-1 genetic variability in Bahia state, Brazil, was investigated. DNA samples from 229 and 213 HIV-1-infected individuals were analyzed using the heteroduplex mobility assay (HMA) in gag and env fragments, respectively. One hundred seventy-five samples were characterized in both genes. Thirty-two subtype F and BF recombinant viruses were sequenced and analyzed by phylogenetic methods. The combination of HMA and sequencing results showed that seven different HIV-1 genotypes comprised this sample: 147 (84%) B/B, 4 (2.3%) F/F, 3 (1.7%) B/F, 1 (0.6%) F/B, 1 (0.6%) F/D, 1 (0.6%) BF/F, and 18 (10.3%) BF/B. A significant divergence was observed between these two techniques results (84.4%). This is explained by the low accuracy of the HMA for detecting recombinant viruses. These recombinants were unrelated to CRF12, while two sequences were related to CRF28 and CRF29. Nineteen BF mosaics shared the same gag breakpoint. In conclusion, the use of HMA may be inappropriate in regions where different subtypes are co-circulating. Subtype B is the most common genotype, however, an increased prevalence (13.1%) of different BF variants and a potentially new CRF suggest that recombination is occurring frequently in Bahia. These viruses were associated with women infected heterosexually. Finally, this study identified the presence of an F/D recombinant HIV-1 in Brazil.
Description: Texto completo: acesso restrito. p. 391–399
URI: http://repositorio.ufba.br/ri/handle/ri/13707
ISSN: 0146-6615
Appears in Collections:Artigos Publicados em Periódicos (Medicina)

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