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Please use this identifier to cite or link to this item: http://repositorio.ufba.br/ri/handle/ri/13701

Title: Effects of freezing on bone histological morphology
Other Titles: Cell and Tissue Banking
Authors: Andrade, Miguel Gustavo Setúbal
Sá, Camila Neves
Marchionni, Antônio Márcio Teixeira
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
Keywords: Bone transplantation;Freezing;Transplantation;Immunology;Necrosis
Issue Date: 2009
Abstract: Allograft bone has been widely used for reconstruction of different portions of the skeleton. The fragment of bone harvested must be kept under low temperatures. The cryopreservation also contributes to decrease the antigenic potential of the tissue. Although this technique is considered safe, there is little information about the morphological modifications that the medullary and cortical portions of bone suffer after freezing. Hence, the aim of this study was to investigate the morphology of bone tissue after freezing under different temperatures and periods. Twelve rabbits were used to analyze the effects of two temperatures, −20°C and −70°C, during four periods of time: 30, 60, 90, 120 days. Tissues were analyzed by HE, picro-sirius stains and also by Feulgen’s reaction, through qualitative and morphometric ways, considering the area occupied by cells and nuclei, medullary and cortical portions, as well as by collagen expression at cortical. The differences among the treatments were analyzed by Tukey´s test, at 5% significance level. Bone freezing increased cellular and nuclear areas at cancellous bone and diminished nuclear area at the cortical bone. Cortical bone collagen suffered denaturation proportionally to temperature decrease and to freezing duration. These alterations compromised the morphology of tissues after 90 or 120 days of freezing at the temperature of −70°C. Cells necrosed during freezing, contributing to reduce bone antigenicity.
Description: Texto completo: acesso restrito. p. 279-287
URI: http://repositorio.ufba.br/ri/handle/ri/13701
ISSN: 1389-9333
Appears in Collections:Artigos Publicados em Periódicos (Odontologia)

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