DSpace Communidade:https://repositorio.ufba.br/handle/ri/205812024-03-25T02:11:36Z2024-03-25T02:11:36ZVariáveis clínicas que interferem na concentração plaquetária do Plasma Rico em Plaquetas (PRP)Pereira, Beatriz Neves da Rochahttps://repositorio.ufba.br/handle/ri/387382023-12-24T01:06:35Z2023-11-21T00:00:00ZTítulo: Variáveis clínicas que interferem na concentração plaquetária do Plasma Rico em Plaquetas (PRP)
Autor(es): Pereira, Beatriz Neves da Rocha
Primeiro Orientador: Daltro, Gildásio de Cerqueira
Abstract: Tissue regeneration is studied a lot within the scientific community and therapeutic options for this, minimally invasive, gain prominence, as they involve much lower intra and postoperative invasive procedures for the patient compared to surgical procedures of great extension. To this end, the autologous biological compound platelet-rich plasma (PRP) becomes a path towards the objective of regenerating an injury at the rate that it delivers to the patient a supraphysiological concentration of cellular mediators found within platelets, active in the inflammatory process, including growth factors and cytokines. The composition of the bioproduct will be influenced by the baseline health of the individual for whom the PRP is being prepared, consequently affecting the development of the therapy. The present work explores some clinical variables that may affect the platelet concentration of the bioproduct by analyzing them using the Poisson distribution, considering studying the relationship between the variable D and PRL with the variables: 𝑋1 = group (1-presence of osteoarthritis; 0- no presence of osteoarthritis); 𝑋2 = gender (1- masculine; 0- feminine); 𝑋3 = age (1- under 60 years old; 0- over 60 years old); 𝑋4 = smoker (1-yes; 0-no); 𝑋5 = alcohol consumption (1-yes; 0-no); 𝑋6 = Frequency of non-steroid anti-inflammatory consumption (NSAIDs) (1- diary; 2- casually; 3- don't use); 𝑋7 = presence of self-reported diabetes (1-yes; 0-no); 𝑋8 = presence of self-reported hypertension (1-yes; 0-no); 𝑋9 = presence of osteoarticular and/or musculoskeletal conditions (1-yes; 0-no). With the exception of the variable gender and frequency of occasional NSAID consumption, the variables had a significant impact on platelet concentration and difference in platelet-rich plasma. Therefore, when determining the production of the biomaterial, it is necessary to give importance and consider the clinical aspects of the patient's health status, observing and analyzing the variables portrayed, since this immunological profile of the patient will also be exposed in the platelet concentrate produced and this will interfere with the therapeutic quality and the desired recovery process.
Editora / Evento / Instituição: Universidade Federal da Bahia
Tipo: Dissertação2023-11-21T00:00:00ZCaracterização genotípica do dengue vírus tipo 1 no estado da BahiaMenezes, Aline Dórea Luzhttps://repositorio.ufba.br/handle/ri/383702023-11-11T05:05:49Z2022-12-14T00:00:00ZTítulo: Caracterização genotípica do dengue vírus tipo 1 no estado da Bahia
Autor(es): Menezes, Aline Dórea Luz
Primeiro Orientador: Campos, Gúbio Soares
Abstract: Dengue is one of the viral diseases transmitted by mosquitoes of the Aedes genus, which occupies a significant place in public health campaigns in Brazil. Four antigenically distinct serotypes are currently known. Factors such as mutations, recombinations, host susceptibility and gene flow of DENV serotypes have caused a greater predisposition to this diversity. In order to investigate the existence of DENV-1 variants in the state of Bahia, in addition to the RT-qPCR technique for the detection of DENV-1, the sequencing technique and phylogenetic analysis of the sequenced regions were used. Analysis of the aligned sequences showed several new SNPs, not present in any of the other strains analyzed.
Editora / Evento / Instituição: Universidade Federal da Bahia
Tipo: Dissertação2022-12-14T00:00:00ZEstabelecimento de técnicas de criopreservação para manutenção do Banco de Microalgas do Instituto de Biologia - Universidade Federal da BahiaSouza, Poline dos Santoshttps://repositorio.ufba.br/handle/ri/376712023-08-19T05:03:48Z2023-07-13T00:00:00ZTítulo: Estabelecimento de técnicas de criopreservação para manutenção do Banco de Microalgas do Instituto de Biologia - Universidade Federal da Bahia
Autor(es): Souza, Poline dos Santos
Primeiro Orientador: Lima, Suzana Telles da Cunha
Abstract: Microalgae are photosynthetic microorganisms found in diverse habitats and capable of producing a high number of compounds of scientific, biotechnological and economic interest. The preservation of the characteristics of microalgae in culture collections has fundamental importance for the study of its biotechnological potential and biodiversity, however, the maintenance of microalgae in a liquid medium can affect the synthesis of compounds of importance for bioprospecting due to mutations, in addition to the risks of contamination. In order to reduce these risks, cryopreservation is an alternative for the maintenance of this material, this technique is widely used in the conservation of microbial biodiversity, which consists of exposing it to cryogenic temperatures, therefore, it is a viable resource for the maintenance of algal species, assisting in the preservation of biochemical characteristics, with a reduction in the growth rate, inbreeding and generating less damage to the genome. The cryopreservation process, however, can cause damage to cells called cryoinjuries, so the use of cryoprotectantes may be necessary. This research aimed to test and adapt cryopreservation protocols for Desmodesmus brasiliensis and Chaetoceros muelleri collected from the Microalgae Bank of the Laboratory of Bioprospection and Biotechnology of the Institute of Biology of the Federal University of Bahia, as well as to evaluate the efficiency and toxicity of cryoprotectants dimethylsulfoxide and methanol in the cryopreservation of the two species and calculate the growth rate for each species. Among the two microalgae tested in the present study, frozen at -80°C for twenty days, it was possible to cryopreserve D. brasiliensis, even without the addition of cryoprotectants, showing the highest growth rate. C. gracilis did not survive the freezing stage, under any of the treatments employed, therefore it is necessary to test new cryoprotectants and cryopreservation methodologies for the species. In view of the results, it is concluded that it is possible to cryopreserve species kept in a liquid medium in biobanks using a species-specific methodology, given their different characteristics.
Editora / Evento / Instituição: Universidade Federal da Bahia
Tipo: Dissertação2023-07-13T00:00:00ZAbordagem Integrada para Predição de Perfis de Resistência a Antimicrobianos à Partir de Dados Genômicos de Isolados Clínicos de Corynebacterium spp.Rocha, Danilo Jobim Passos Gil dahttps://repositorio.ufba.br/handle/ri/374782023-08-05T05:03:39Z2023-05-30T00:00:00ZTítulo: Abordagem Integrada para Predição de Perfis de Resistência a Antimicrobianos à Partir de Dados Genômicos de Isolados Clínicos de Corynebacterium spp.
Autor(es): Rocha, Danilo Jobim Passos Gil da
Primeiro Orientador: Pacheco, Luis Gustavo Carvalho
Abstract: Antimicrobial resistance represents a threat to global public health. The rapid spread of multidrug-resistant pathogenic bacteria and the inability of antibiotics to effectively combat them have led to more severe and prolonged infections. To mitigate this challenge, whole-genome sequencing has been proposed as a tool for epidemiological surveillance and as an alternative to phenotypic tests for predicting resistance, with the promise of rapid, accurate results and better-targeted treatment. However, its application for the latter faces significant obstacles. For clinically important organisms such as Mycobacterium tuberculosis, some studies can establish up to 99% concordance between
genotype and antibiotic susceptibility phenotype. On the other hand, other microorganisms, such as emerging pathogens of the Corynebacterium genus, are far from achieving an acceptable level of concordance in clinical application, reflecting a lack of understanding of the microorganism and its resistance mechanisms. In this work, we sequenced the multidrug-resistant isolate VH4248 from the Hospital Marqués de Valdecillla in Santander, Spain, identified as C. urealyticum by classical microbiological and VITEK1 methods. Genome-based identification tools established only a 93.7% similarity with the C. urealyticum database, making reclassification to Corynebacterium sp. necessary. We also assembled, annotated, and explored the genomes of 107 publicly available C. striatum isolates using the PATRIC (BV-BRC), KmerResistance, and Resfinder tools for genomic prediction of antibiotic resistance to beta-lactams, aminoglycosides, macrolides, tetracyclines, and fluoroquinolones. We compared the results with phenotypic data through ROC curves and identified the widely distributed ermX, tetW, and blaA genes with ROC curve areas (AUC) > 0.874 for erythromycin, tetracycline, and penicillin antibiotics. However, all prediction tools showed suboptimal capacity for predicting resistance in C. striatum when evaluated according to international parameters: major error rates (MERs) and very major error rates (VMERs). Accordingly, when we evaluated new clinical isolates of Corynebacterium spp. (n = 18) by polymerase chain reaction (PCR) using primer oligonucleotides for the major identified antibiotic resistance genes, only tetW and ermX genes showed good levels of concordance with phenotypic antibiotic susceptibility test results (94.1% and 83.3%, respectively). Therefore, we conclude that further studies are needed to standardize and establish efficient strategies for resistance prediction based on genomic data from clinically relevant Corynebacterium spp.
Editora / Evento / Instituição: Universidade Federal da Bahia
Tipo: Tese2023-05-30T00:00:00Z